Anti-OGDH antibody [HL2072] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OGDH antibody [HL2072] - BSA and Azide free (AB327535)

ab327535 detects OGDH protein at mitochondria by immunofluorescent analysis.
Sample : HeLa cells were fixed in ice-cold MeOH for 5 min.
Green : OGDH stained by ab327535 diluted at 1 : 500.
Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1 : 1000.
Blue : Fluoroshield with DAPI

• WB

Supplier Data

Western blot - Anti-OGDH antibody [HL2072] - BSA and Azide free (AB327535)

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab327535 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-OGDH antibody [HL2072] - BSA and Azide free (ab327535) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-OGDH antibody [HL2072] - BSA and Azide free (AB327535)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab327535 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-OGDH antibody [HL2072] - BSA and Azide free (ab327535) at 1/5000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged OGDH transfected (+) 293T whole cell extracts at 30 µg

Lane 3:

Myc-DDK-tagged OGDHL transfected (+) 293T at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-OGDH antibody [HL2072] - BSA and Azide free (AB327535)

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab327535 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-OGDH antibody [HL2072] - BSA and Azide free (ab327535) at 1/1000 dilution

Lane 1:

Mouse kidney tissue extracts at 50 µg

Lane 2:

Mouse muscle tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-OGDH antibody [HL2072] - BSA and Azide free (AB327535)

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ab327535 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-OGDH antibody [HL2072] - BSA and Azide free (ab327535) at 1/1000 dilution

All lanes:

Rat muscle tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false