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AB276046

Anti-OLFM4 antibody [12] - BSA and Azide free

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Mouse Monoclonal OLFM4 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human OLFM4.

대체 명칭 보기

GW112, UNQ362/PRO698, OLFM4, Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hOLfD, hGC-1

2 이미지
Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)

Intracellular flow cytometric analysis of OLFM4 expression in DU145 cells. The cells were stained with ab276046 at 1/25 dilution, then a FITC-conjugated second step antibody. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)
  • ICC

Unknown

Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)

Immunofluorescence staining of OLFM4 in A431 (human epidermoid carcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276046 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with an AlexaFluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

12

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

ICC, Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human OLFM4.

Q6UX06

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보완 제품

Assay Kits

AB267805

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Primary Antibodies

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Primary Antibodies

AB180515

Anti-alpha Defensin 5 antibody [EPR14309(B)]

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  • 0
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Primary Antibodies

AB283265

Anti-Chromogranin A antibody [RM1025]

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Flow Cytometry (Intracellular) - Anti-Chromogranin A antibody [RM1025] (AB283265)

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Primary Antibodies

AB180851

Anti-CLCA1 antibody [EPR12254-88]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (AB180851)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/25 - 1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The OLFM4 protein also known as Olfactomedin 4 or GW112 has a molecular weight of around 57 kDa. It is a glycoprotein expressed mainly in human and some non-human primate tissues including the prostate and gastrointestinal tract. OLFM4 is a secreted protein found in various cells including those of the bone marrow and esophagus. It plays a role in innate immunity and is a part of the olfactomedin family which is involved in cell adhesion and migration processes.
Biological function summary

OLFM4 functions in inhibiting bacterial growth by interfering with bacterial adhesion and survival. It is not part of a larger protein complex but interacts with cell surface proteins to exert its effects. OLFM4 acts in the regulation of cell differentiation and growth playing a significant part in maintaining the stem cell milieu in the intestine.

Pathways

OLFM4 participates in cellular defense mechanisms primarily against microbial pathogens by involving in the activation of immune response pathways. It is associated with pathways such as the NF-kB signaling and Janus kinase (JAK) pathways. Through these pathways OLFM4 might interact with cytokines and other inflammatory mediators or modulators.

OLFM4 relates to cancer particularly gastric and colorectal cancer where its expression levels serve as potential biomarkers for disease progression. Additionally it associates with inflammatory bowel diseases impacting the mucosal immune response. In cancer OLFM4's interaction with proteins like MMP isoforms (matrix metalloproteinases) can influence tumor metastasis and invasiveness.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.
See full target information OLFM4
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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