AB230626

Anti-Olig3 antibody

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Rabbit Polyclonal OLIG3 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OLIG3 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

BHLHB7, BHLHE20, OLIG3, Oligodendrocyte transcription factor 3, Oligo3, Class B basic helix-loop-helix protein 7, Class E basic helix-loop-helix protein 20, bHLHb7, bHLHe20

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Flow Cytometry (Intracellular) - Anti-Olig3 antibody (AB230626)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Olig3 antibody (AB230626)

Intracellular flow cytometric analysis ofU-251 MG (human brain glioma cell line) cell line labeling Olig3 with ab230626 at 1/10 dilution (green) compared with a negative control cell (blue).

FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig3 antibody (AB230626)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig3 antibody (AB230626)

Formalin-fixed, paraffin-embedded human skin tissue stained for Olig3 using ab230626 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig3 antibody (AB230626)

Formalin-fixed, paraffin-embedded human testis tissue stained for Olig3 using ab230626 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Olig3 antibody (AB230626)

All lanes:

Western blot - Anti-Olig3 antibody (ab230626) at 1/1000 dilution

All lanes:

Mouse spleen lysate at 35 µg

Predicted band size: 29 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human OLIG3 aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q7RTU3

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Olig3 also known as Oligodendrocyte transcription factor 3 acts as a basic helix-loop-helix (bHLH) transcription factor. It has a mass of about 31 kDa and plays an important role in regulating gene expression. You typically find it expressed in developing neural tissues especially in the central nervous system. In humans it is located on chromosome 6 where its precise control is necessary for proper neural development.

Biological function summary

Olig3 regulates the development of specific neurons in the central nervous system. It does not operate alone but often forms heterodimers with other bHLH transcription factors to exert its influence. Researchers have shown its involvement in neurogenesis particularly in defining neuronal cell fate. By activating or repressing specific genes it fine-tunes the development of neural progenitor cells into specialized neuron types.

Pathways

Olig3 integrates into the Notch and Sonic Hedgehog signaling pathways. It influences neuronal differentiation through these pathways often interacting with proteins like Hes1 which is part of the Notch pathway. Sonic Hedgehog also plays a role in guiding the spatial expression patterns where Olig3 is active ensuring a balanced developmental process. These pathways are essential for the correct propagation of signals that dictate the specification of neuronal cell lineages.

Olig3 has been implicated in medulloblastoma and certain developmental disorders. Dysregulation of Olig3 expression and function can contribute to tumor formation in the cerebellum often in conjunction with associated proteins such as Gli1 from the Sonic Hedgehog pathway. Abnormalities in Olig3 expression may also link to developmental neuroissues due to its foundational role in neuron formation and differentiation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.

See full target information OLIG3

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer research 83:3783-3795 PubMed37668527

2023

A Cell Surface-Binding Antibody Atlas Nominates a MUC18-Directed Antibody-Drug Conjugate for Targeting Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Shi,Tao Jiao,Qian Guo,Weining Weng,Linjie Ma,Qing Zhang,Lijun Wang,Jianjian Zhang,Caiwei Chen,Yaling Huang,Mingqiao Wang,Rong Pan,Yanfang Tang,Wenhao Hu,Tao Meng,Shu-Hui Liu,Jun Guo,Yan Kong,Xun Meng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Olig3 antibody

Flow Cytometry (Intracellular) - Anti-Olig3 antibody (AB230626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig3 antibody (AB230626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig3 antibody (AB230626)

Western blot - Anti-Olig3 antibody (AB230626)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise