Anti-OMA1 antibody [EPR29198-78] - BSA and Azide free

• WB

Lab

Western blot - Anti-OMA1 antibody [EPR29198-78] - BSA and Azide free (AB326340)

This data was developed using ab324586, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 24719224).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-OMA1 antibody [EPR29198-78] (<a href='/ko/products/primary-antibodies/oma1-antibody-epr29198-78-ab324586'>ab324586</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting OMA1 whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-OMA1 antibody [EPR29198-78] - BSA and Azide free (AB326340)

This data was developed using ab324586, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot Anti-COX IV antibody [EPR9442 (ABC)] - Mitochondrial Loading Control (ab202554) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-OMA1 antibody [EPR29198-78] (<a href='/ko/products/primary-antibodies/oma1-antibody-epr29198-78-ab324586'>ab324586</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) mitochondria fraction at 20 µg

Lane 2:

HeLa non-mitochondrial fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 36 kDa,16 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-OMA1 antibody [EPR29198-78] - BSA and Azide free (AB326340)

This data was developed using ab324586, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands higher than 100 kDa are unknown.

Lanes 1-2 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross reactivity with human IgG at 1/2000 and lanes 3-4 are applied with Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/20000

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-OMA1 antibody [EPR29198-78] (<a href='/ko/products/primary-antibodies/oma1-antibody-epr29198-78-ab324586'>ab324586</a>) at 1/1000 dilution

Lanes 1 - 2:

Human heart tissue lysate at 20 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

SK-MEL-28 (human malignant melanoma cell ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 36 kDa

false

Exposure time: 180s