Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free
- RabMAb
- Recombinant
- 각 태그의 뜻
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(2 제품이 사용된 논문 )
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (AB240143)
Immunohistochemical analysis of Paraffin-embedded Human brain tissue labeling OPA1 with ab157457 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (AB240143)
Immunohistochemical analysis of Paraffin-embedded Human retina tissue labeling OPA1 with ab157457 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-Fr
Unknown
Immunohistochemistry (Frozen sections) - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (AB240143)
Immunohistochemistry (Frozen sections) analysis of mouse retina tissue sections labeling OPA1 with Purified ab157457 at 1/250 (1.1 μg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. DAPI was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157457).
- WB
Lab
Western blot - Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (AB240143)
This data was developed using ab157457, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.
All lanes:
Western blot - Anti-OPA1 antibody [EPR11057(B)] (<a href='/ko/products/primary-antibodies/opa1-antibody-epr11057b-ab157457'>ab157457</a>) at 1/1000 dilution
Lane 1:
SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
MEF (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 3:
Rat retina tissue lysate at 20 µg
Lane 4:
Human eyeball tissue lysate at 20 µg
Lane 5:
Mouse eyeball tissue lysate at 20 µg
Lane 6:
Mouse brain tissue lysate at 20 µg
Lane 7:
Rat brain tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 80-100 kDa
false
Exposure time: 100s
관련 conjugated 항체와 다양한 조성의 항체 (1)
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Anti-OPA1 antibody [EPR11057(B)]
Reactivity 정보
제품 세부 정보
ab240143 is the carrier-free version of ab157457.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Mitochondrial dynamics involving OPA1 ensure energy production efficiency and cell health. OPA1 plays a role in mitochondrial fusion by forming a complex with mitofusins MFN1 and MFN2. This complex maintains the integrity of mitochondrial networks facilitates proper respiratory function and prevents apoptosis by regulating cristae junctions. It also participates in the stress response particularly in the preservation of the mitochondrial structure and function under challenging conditions.
Pathways
OPA1 integrates into the mitochondrial fusion and fission pathways important for cellular energy metabolism. It works alongside proteins like DRP1 in balancing these processes. The involvement in these pathways is essential for cellular adaptation to metabolic needs and stress. OPA1 also has a relationship with the PINK1/Parkin pathway where its regulation affects mitophagy a process of clearing damaged mitochondria. These interactions highlight the importance of OPA1 in maintaining cellular and mitochondrial homeostasis.
제품 프로토콜
- Visit the General protocols
- Visit the Troubleshooting
타겟 정보
대체 명칭 보기
제품이 사용된 논문 (2)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Bio-protocol 15:e5424 PubMed40873476
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cells 13: PubMed39273021
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com