AB85830

Anti-ORC1 antibody

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(9 제품이 사용된 논문 )

Rabbit Polyclonal ORC1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ORC1 aa 800 to C-terminus.

2 이미지

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Immunoprecipitation - Anti-ORC1 antibody (AB85830)

Detection of Human ORC1 by Western Blot of Immunprecipitate.
All lanes : ab85830 at 1µg/ml staining ORC1 in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies :
Lane 1 : ab85830
Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ORC1 antibody (ab85830)

Predicted band size: 97 kDa

false

Unknown

Western blot - Anti-ORC1 antibody (AB85830)

All lanes:

Western blot - Anti-ORC1 antibody (ab85830) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 97 kDa

Observed band size: 107 kDa

false

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ORC1 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13415

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab85830 was affinity purified using an epitope specific to ORC1 immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ORC1 also known as Origin Recognition Complex subunit 1 is an important component involved in the initiation of DNA replication. It forms part of a larger protein complex that plays a role in recognizing the starting points or origins for replication to begin. ORC1 has a molecular mass of approximately 97 kDa and is expressed in various cell types throughout the body but is especially prominent in cells that actively divide. Its expression ensures that the replication machinery assembles correctly during the cell cycle.

Biological function summary

In terms of chromosomal DNA replication ORC1 plays a central role in forming the pre-replicative complex. This protein is a component of the ORC complex which includes several other subunits responsible for facilitating replication initiation across eukaryotic cells. ORC1 through interactions with additional proteins like CDC6 and CDT1 helps load the MCM helicase onto DNA which is essential for DNA unwinding before replication.

Pathways

The replication origins and ORC1's role in them tie it to the DNA replication and cell cycle pathways. In the DNA replication pathway ORC1 serves as the initial marker for DNA segment to act as a template for the replication machinery. Additionally its relationship with CDC6 links it to the cell cycle regulatory pathways orchestrating timely DNA replication to ensure that cell division occurs accurately and efficiently.

Defects or dysregulation in ORC1 are linked to Meier-Gorlin syndrome a condition characterized by growth delays and developmental abnormalities. Further irregularities in ORC1 function can also contribute to certain cancers due to uncontrolled cell division. Within these disorders connections between ORC1 and proteins such as CDT1 emphasize its role in ensuring orderly cell cycle progression whose disruption can lead to disease pathogenesis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

See full target information ORC1

대체 명칭 보기

ORC1L, PARC1, ORC1, Origin recognition complex subunit 1, Replication control protein 1

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록and refine your search

The Journal of clinical investigation 135: PubMed40454487

2025

The class II myosin MYH4 safeguards genome integrity and suppresses tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jayashree Thatte,Ana Moisés da Silva,Judit Börcsök,Thorkell Gudjónsson,Jan Benada,Xin Li,Muthiah Bose,Hanneke van der Gulden,Ji-Ying Song,Renée Menezes,Elena Martín-Doncel,Luis Toledo,Valdemaras Petrosius,Cord Brakebusch,Jos Jonkers,Finn Cilius Nielsen,Maria Rossing,Claus S Sørensen

Journal of virology 98:e0003524 PubMed39082875

2024

ORC1 enhances repressive epigenetic modifications on HIV-1 LTR to promote HIV-1 latency.

Applications

Unspecified application

Species

Unspecified reactive species

Mo Zhou,Tao Yang,Ming Yuan,Xinyu Li,Jieyi Deng,Shiyu Wu,Zhihan Zhong,Yingtong Lin,Wanying Zhang,Baijin Xia,Yating Wu,Lilin Wang,Tao Chen,Ruxin Liu,Ting Pan,Xiancai Ma,Linghua Li,Bingfeng Liu,Hui Zhang

Nature communications 14:5076 PubMed37604829

2023

NFIB facilitates replication licensing by acting as a genome organizer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Zhang,Yue Wang,Yongjie Liu,Cuifang Liu,Yizhou Wang,Lin He,Xiao Cheng,Yani Peng,Lu Xia,Xiaodi Wu,Jiajing Wu,Yu Zhang,Luyang Sun,Ping Chen,Guohong Li,Qiang Tu,Jing Liang,Yongfeng Shang

Oncology letters 24:252 PubMed35761947

2022

Origin recognition complex 1 regulates phospholipase Cδ1 to inhibit cell proliferation, migration and epithelial-mesenchymal transition in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Jian,Qing Qiao,Juanjuan Tang,Xiaobing Qin

Signal transduction and targeted therapy 7:102 PubMed35414135

2022

LSD1 is required for euchromatic origin firing and replication timing.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Wang,Yunchao Huang,Edith Cheng,Xinhua Liu,Yu Zhang,Jianguo Yang,Jordan T F Young,Grant W Brown,Xiaohan Yang,Yongfeng Shang

Oncology reports 42:2670-2679 PubMed31545502

2019

Knockdown of Nedd8‑conjugating enzyme UBE2M suppresses the proliferation and induces the apoptosis of intrahepatic cholangiocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhao,Caiyan Gao,Daixiu Shi,Jie Mao,Jun Zhao,Lingyun Guo,Jiwu Guo,Zuoyi Jiao

Cancer cell international 19:45 PubMed30858762

2019

Up-regulated lncRNA XIST contributes to progression of cervical cancer via regulating miR-140-5p and .

Applications

Unspecified application

Species

Unspecified reactive species

Xing Chen,Dongsheng Xiong,Liya Ye,Kai Wang,Lingfei Huang,Shuangshuang Mei,Jinhong Wu,Shanshan Chen,Xiaoli Lai,Lingzhi Zheng,Meifen Wang

Cell death & disease 5:e1351 PubMed25058425

2014

Immortalised breast epithelia survive prolonged DNA replication stress and return to cycle from a senescent-like state.

Applications

Species

Human

A Maya-Mendoza,J M Merchut-Maya,J Bartkova,J Bartek,C H Streuli,D A Jackson

The Journal of biological chemistry 287:15024-33 PubMed22427655

2012

Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing.

Applications

Species

Unspecified reactive species

Kui Ming Chan,Zhiguo Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ORC1 antibody

Immunoprecipitation - Anti-ORC1 antibody (AB85830)

Western blot - Anti-ORC1 antibody (AB85830)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (9)

Product promise