AB9641

Anti-ORC4L antibody

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(11 제품이 사용된 논문 )

Goat Polyclonal ORC4L antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human ORC4 aa 400 to C-terminus.

1 이미지

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Western blot - Anti-ORC4L antibody (AB9641)

All lanes:

Western blot - Anti-ORC4L antibody (ab9641) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 50 kDa

Observed band size: 38 kDa,49 kDa

true

Exposure time: 20min

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human ORC4 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43929

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The origin recognition complex subunit 4-like (ORC4L) also known simply as ORC4 is a mechanically important player in the initiation of DNA replication. ORC4 typically has a molecular mass of approximately 50 kDa. It is highly expressed in cells that are actively dividing especially within the nucleus where replication origins are located. The protein forms part of the larger origin recognition complex (ORC) which is important for marking the starting points for DNA replication across eukaryotic organisms.

Biological function summary

ORC4 plays a central role in recognizing replication origins and acts as an anchor for the assembly of other necessary replication proteins. It is one of six subunits that comprise the ORC complex. This protein complex binds to origins of replication in DNA unwinds the double helix and recruits additional factors required for the formation of the pre-replicative complex. The presence of ORC4 is essential in ensuring that DNA replication occurs once and only once per cell cycle preventing genomic instability.

Pathways

Several cellular processes depend on the correct functioning of ORC4. In cell cycle regulation ORC4 is integral to the DNA replication checkpoint which ensures DNA is not damaged before it is duplicated. ORC4 interacts with other replication factors like CDC6 and MCM2-7 helicase to initiate and regulate the replication process. Its activity influences key pathways such as the p53-dependent DNA damage response where ORC4 can affect decisions related to cell cycle arrest or apoptosis.

ORC4 dysregulation links to genomic instability which is a hallmark in the development of cancer. Altered levels or mutations in ORC4 contribute to an increased susceptibility to oncogenesis with links to various cancers such as those of the breast and colon. The BRCA1 protein known for its role in maintaining genomic stability and DNA repair often shows interactions with ORC4 during such disease states highlighting their interconnected roles in cancer-related pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.

See full target information ORC4

대체 명칭 보기

ORC4L, ORC4, Origin recognition complex subunit 4

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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular cell 81:1951-1969.e6 PubMed33761311

2021

Multiple, short protein binding motifs in ORC1 and CDC6 control the initiation of DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

Manzar Hossain,Kuhulika Bhalla,Bruce Stillman

Cell cycle (Georgetown, Tex.) 18:605-620 PubMed30741601

2019

Human NOC3 is essential for DNA replication licensing in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Man-Hei Cheung,Aftab Amin,Rentian Wu,Yan Qin,Lan Zou,Zhiling Yu,Chun Liang

Oncotarget 7:80190-80207 PubMed27863397

2016

Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing.

Applications

ICC/IF

Species

Human

Ruowen Zhang,Jiahui Wu,Sylvain Ferrandon,Katie J Glowacki,Janet A Houghton

eLife 5: PubMed27458800

2016

Opposing roles for DNA replication initiator proteins ORC1 and CDC6 in control of Cyclin E gene transcription.

Applications

Species

Human

Manzar Hossain,Bruce Stillman

PloS one 10:e0128497 PubMed26038842

2015

Mapping of Replication Origins in the X Inactivation Center of Vole Microtus levis Reveals Extended Replication Initiation Zone.

Applications

Species

Unspecified reactive species

Vladimir V Sherstyuk,Alexander I Shevchenko,Suren M Zakian

The Journal of biological chemistry 287:15024-33 PubMed22427655

2012

Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing.

Applications

Species

Unspecified reactive species

Kui Ming Chan,Zhiguo Zhang

The Journal of biological chemistry 286:23831-41 PubMed21555516

2011

Assembly of the human origin recognition complex occurs through independent nuclear localization of its components.

Applications

Species

Human

Soma Ghosh,Alex P Vassilev,Junmei Zhang,Yingming Zhao,Melvin L DePamphilis

The Journal of biological chemistry 283:2265-74 PubMed18029353

2007

FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.

Applications

Species

Human

Yoshikazu Johmura,Shigehiro Osada,Makoto Nishizuka,Masayoshi Imagawa

The Journal of biological chemistry 281:23264-73 PubMed16762929

2006

Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex.

Applications

Unspecified application

Species

Unspecified reactive species

Ilian Radichev,Sung Won Kwon,Yingming Zhao,Melvin L DePamphilis,Alex Vassilev

Molecular cell 19:567-75 PubMed16109380

2005

Human origins of DNA replication selected from a library of nascent DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Vesna Todorovic,Sara Giadrossi,Cristina Pelizon,Ramiro Mendoza-Maldonado,Hisao Masai,Mauro Giacca

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ORC4L antibody

Western blot - Anti-ORC4L antibody (AB9641)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (11)

Product promise