AB244243

Anti-Ornithine Carbamoyltransferase/OTC antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal Ornithine Carbamoyltransferase/OTC antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Ornithine transcarbamylase, mitochondrial aa 150-300.

대체 명칭 보기

OTCase, OTC

9 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Ornithine Carbamoyltransferase/OTC with ab244243 at a 1/2500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for Ornithine Carbamoyltransferase/OTC with ab244243 at a 1/2500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for Ornithine Carbamoyltransferase/OTC with ab244243 at a 1/2500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Formalin-fixed, paraffin-embedded human testis tissue stained for Ornithine Carbamoyltransferase/OTC with ab244243 at a 1/2500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling Ornithine Carbamoyltransferase/OTC using ab244243 at 4 μg/ml (green) in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for Ornithine Carbamoyltransferase/OTC with ab244243 at a 1/2500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Formalin-fixed, paraffin-embedded human liver tissue stained for Ornithine Carbamoyltransferase/OTC with ab244243 at a 1/2500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Ornithine Carbamoyltransferase/OTC with ab244243 at a 1/2500 dilution in immunohistochemical analysis.

No positivity as expected.

Supplier Data

Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

All lanes:

Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody (ab244243) at 0.4 µg/mL

All lanes:

Human liver tissue lysate

Predicted band size: 39 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Ornithine transcarbamylase, mitochondrial aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

P00480

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ornithine Carbamoyltransferase (OTC) also known as ornithine aminotransferase is an enzyme critical in the urea cycle. The OTC protein facilitates the conversion of ornithine and carbamoyl phosphate into citrulline a process essential for nitrogen excretion. The human OTC protein has a mass of approximately 36 kilodaltons and is primarily expressed in the liver mitochondria. Protein assays and procurement efforts often focus on obtaining accurate measurements and ensuring high-purity OTC peptides enabling proper functional studies.

Biological function summary

Ornithine carbamoyltransferase plays a significant role in detoxifying ammonia by forming citrulline an important urea cycle intermediate. The protein functions as a homotrimer meaning three identical subunits form an active enzyme complex. This multimeric state facilitates efficient substrate processing allowing effective handling of nitrogen waste. The integral role of OTC in the urea cycle highlights its importance in maintaining systemic nitrogen balance.

Pathways

OTC is a central component of the urea cycle and influences nitrogen metabolism pathways. These pathways include the conversion of ammonia to urea an important detoxifying process for safe excretion by the kidneys. OTC closely interacts with enzymes such as argininosuccinate synthetase highlighting the coordinated action required to advance urea formation. Understanding OTC's participation within these pathways helps identify its regulatory points and the potential impact of genetic variations.

Mutations in the OTC gene can lead to ornithine transcarbamylase deficiency a disorder affecting ammonia detoxification. This X-linked genetic condition disrupts normal nitrogen metabolism causing ammonia accumulation particularly harmful to the central nervous system. OTC deficiency shares a functional relationship with other urea cycle disorders such as citrullinemia due to its contribution to the same biochemical pathway. Researchers focus on identifying OTC protein mutations to improve diagnosis and treatment of these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline (PubMed : 2556444, PubMed : 6372096, PubMed : 8112735). The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion (PubMed : 2556444).

See full target information Ornithine transcarbamylase, mitochondrial

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of experimental & clinical cancer research : CR 44:239 PubMed40813699

2025

Arginine depletion potentiates standard-of-care chemo-immunotherapy in preclinical models of high-risk neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberley M Hanssen,Jayne Murray,Ruby Pandher,Stephanie Alfred,Laura D Gamble,Jennifer Brand,Erin Mosmann,Frances K Kusuma,Crystal Mak,Adam Kearns,Alvin Kamili,Caroline Atkinson,Alexis Z Minchaca,Jean Bertoldo,David S Ziegler,Francis Mussai,Paul N M Cheng,Murray D Norris,Jamie I Fletcher,Michelle Haber

International journal of molecular sciences 23: PubMed35457188

2022

Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Shanshan Cui,Ling Hao,Wen Liu,Lijing Wang,Mengwei Ju,Wenjing Feng,Rong Xiao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ornithine Carbamoyltransferase/OTC antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise