AB322041

Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6]

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal Orosomucoid 2 antibody. Suitable for Dot, WB and reacts with Recombinant full length protein - Human, Recombinant fragment - Human, Human samples.

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Supplier Data

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed Mw of ~45KDa is higher than the predicted Mw due to glycosylation (PMID : 33070381).

All lanes:

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (ab322041) at 1/1000 dilution

All lanes:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 45 kDa

false

Exposure time: 114s

Supplier Data

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, skeletal muscle(PMID : 20442402)

The observed Mw of ~45KDa is higher than the predicted Mw due to glycosylation (PMID : 33070381).

The identity of the lower MW band at approximately 18 kDa (in lane 1) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (ab322041) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 45 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Dot Blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Dot blot analysis of Orosomucoid 1 + Orosomucoid 2 using ab322041 at 1 : 1000 (0.508 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 10,000 dilution.

Lane1 : His-tagged human ORM1 protein
Lane2 : His-tagged human ORM2 protein

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (ab322041) at 1/1000 dilution

Lane 1:

His-tagged human ORM1 protein at 5 ng

Lane 2:

His-tagged human ORM2 protein at 5 ng

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26603-6

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Acid Glycoprotein also known as AGP or Orosomucoid 2 is a plasma protein with a molecular mass of around 40-45 kDa. This glycoprotein is rich in carbohydrate content with about 45% of its mass made up of glycan chains. It is primarily synthesized in the liver and circulates in the bloodstream. AGP functions as a major acute-phase protein meaning its levels increase in response to inflammation or tissue injury.

Biological function summary

AGP has significant roles in modulating immune responses and transport functions. This molecule interacts with various drugs and hormones due to its binding capacity thereby influencing their bioavailability and distribution. It forms part of a complex dynamic system that helps modulate the body's response to acute inflammatory stimuli. As a glycoprotein AGP plays a role in processes such as cell-to-cell communication and cell signaling which are essential for maintaining homeostasis during stress responses.

Pathways

AGP is integrally involved in the acute-phase response pathway that gets activated during infection or injury. This pathway leads to the dramatic increase of AGP and other acute-phase proteins like C-reactive protein (CRP) in the blood. Furthermore AGP is related to the immune response pathways where it can modulate the activity of various immune cells. Its binding interactions with cytokines and other inflammatory modulators emphasize its role in regulating inflammation and immune responses.

AGP levels often correlate with conditions involving acute and chronic inflammation like rheumatoid arthritis and cancer. Elevated AGP levels are frequently observed in patients with these disorders and reflect the body's ongoing inflammatory response. The increase of AGP in these diseases often correlates with other proteins such as serum amyloid A (SAA) and CRP which serve similar functions in the acute-phase response. This correlation with other inflammatory markers highlights AGP's importance in understanding and potentially diagnosing inflammatory conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Functions as a transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.

See full target information ORM2

Additional targets

ORM1

대체 명칭 보기

AGP2, ORM2, Alpha-1-acid glycoprotein 2, AGP 2, Orosomucoid-2, OMD 2, AGP1, ORM1, Alpha-1-acid glycoprotein 1, AGP 1, Orosomucoid-1, OMD 1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6]

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Dot Blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Assay Kits

Proteins & Peptides

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

대체 명칭 보기

Product promise