AB59016

Anti-OSBPL5/ORP5 antibody

Name: Anti-OSBPL5/ORP5 antibody
Brand: Abcam Limited
SKU: AB59016
Rating: 1 (1 reviews)

(1 리뷰)

(7 제품이 사용된 논문 )

Goat Polyclonal OSBPL5/ORP5 antibody. Suitable for Flow Cyt, ICC/IF, IHC-P, WB and reacts with Human, Rat samples. Cited in 7 publications.

5 이미지

Flow Cytometry - Anti-OSBPL5/ORP5 antibody (AB59016)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-OSBPL5/ORP5 antibody (AB59016)

ab59016 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB59016)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB59016)

ab59016 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB59016)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB59016)

ab59016 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB59016)

ab59016 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB59016)

ab59016 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is expected to recognise both reported isoforms (NP_065947.1; NP_663613.1).

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.3 µg/mL", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.3 µg/mL", "WB-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

OSBPL5 also known as ORP5 is a member of the oxysterol-binding protein (OSBP) family. This protein weighs approximately 99 kDa and is expressed in various tissues including the liver and the brain. OSBPL5 functions primarily as a lipid transfer protein playing a mechanical role in maintaining lipid homeostasis at membrane contact sites. It facilitates the transfer of phosphatidylserine and phosphatidylinositol-4-phosphate between membranes which is essential for cellular lipid regulation.

Biological function summary

The OSBP-like protein OSBPL5 is involved in cellular processes such as membrane trafficking and signal transduction. OSBPL5 is a part of the larger OSBP-related protein complex which regulates lipid interactions and distribution in cells. It influences vesicular transport and is implicated in the modulation of signaling pathways that govern cell growth and development.

Pathways

OSBPL5 functions in the phosphoinositide signaling and lipid metabolic pathways. It plays an important role in the transfer of lipids between the endoplasmic reticulum and other organelles impacting the regulation of phosphatidylinositol at the plasma membrane. OSBPL5 interacts with proteins like PI4KIIIα to regulate these processes. This interaction is important for maintaining cellular lipid dynamics necessary for various physiological functions.

OSBPL5's lipid transfer function associates with metabolic disorders like non-alcoholic fatty liver disease (NAFLD). Disruptions in lipid homeostasis by OSBPL5 can lead to excessive lipid accumulation in liver cells. Additionally OSBPL5 may have implications in neurological disorders given its expression in the brain alongside proteins such as APP that are involved in similar pathways. Understanding its role could provide insights into these diseases potentially aiding in therapeutic interventions.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane : specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed : 23934110, PubMed : 26206935). May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes (PubMed : 21220512). Binds 25-hydroxycholesterol and cholesterol (PubMed : 17428193).

See full target information OSBPL5

대체 명칭 보기

KIAA1534, OBPH1, ORP5, OSBPL5, Oxysterol-binding protein-related protein 5, ORP-5, OSBP-related protein 5, Oxysterol-binding protein homolog 1

style

left-column

제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell stress & chaperones 28:395-407 PubMed37314629

2023

ORP5 promotes migration and invasion of cervical cancer cells by inhibiting endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wu,Luyao Min,Peng Zhang,Lin Zhang,Yuting Xu,Danhua Li,Maojin Zheng,Dongsheng Pei,Qingling Wang

Cell death discovery 8:219 PubMed35449154

2022

ORP5 promotes tumor metastasis via stabilizing c-Met in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Li Song,Lin Zhang,Yun Zhou,Xiaotong Shao,Yuting Xu,Dongsheng Pei,Qingling Wang

BMC pulmonary medicine 22:30 PubMed35000595

2022

The LMCD1-AS1/miR-526b-3p/OSBPL5 axis promotes cell proliferation, migration and invasion in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Hu,Yankai Yu,Haining Wang

Journal of immunology (Baltimore, Md. : 1950) 204:1134-1145 PubMed31953353

2020

OSBP-Related Protein 5L Maintains Intracellular IP/Ca Signaling and Proliferation in T Cells by Facilitating PIP Hydrolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyang Xu,Biying Zhu,Xiuye Cao,Shannai Li,Dan Li,Huihao Zhou,Vesa M Olkkonen,Wenbin Zhong,Jun Xu,Daoguang Yan

The Journal of cell biology 217:1797-1813 PubMed29472386

2018

PI(4,5)P controls plasma membrane PI4P and PS levels via ORP5/8 recruitment to ER-PM contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Mira Sohn,Marek Korzeniowski,James P Zewe,Rachel C Wills,Gerald R V Hammond,Jana Humpolickova,Lukas Vrzal,Dominika Chalupska,Vaclav Veverka,Gregory D Fairn,Evzen Boura,Tamas Balla

The Journal of biological chemistry 293:3806-3818 PubMed29358326

2018

Oxysterol-binding protein-related protein 5 (ORP5) promotes cell proliferation by activation of mTORC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ximing Du,Armella Zadoorian,Ivan E Lukmantara,Yanfei Qi,Andrew J Brown,Hongyuan Yang

Nature communications 8:757 PubMed28970484

2017

ORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate (PtdIns(4,5)P ) and regulate its level at the plasma membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Rajesh Ghai,Ximing Du,Huan Wang,Jiangqing Dong,Charles Ferguson,Andrew J Brown,Robert G Parton,Jia-Wei Wu,Hongyuan Yang

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OSBPL5/ORP5 antibody

Flow Cytometry - Anti-OSBPL5/ORP5 antibody (AB59016)

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB59016)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB59016)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB59016)

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB59016)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (7)

Product promise