JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB269411

Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (Detector)

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal Osteopontin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
1 이미지
Sandwich ELISA - Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (Detector) (AB269411)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (Detector) (AB269411)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab269374).

관련 conjugated 항체와 다양한 조성의 항체 (8)

  • Unconjugated

    Anti-Osteopontin antibody [EPR21139-316]

  • Rat version

    Anti-Osteopontin antibody [EPR21139-316] - Rat IgG2a (Chimeric)

  • Carrier free

    Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Osteopontin antibody [EPR21139-316]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Osteopontin antibody [EPR21139-316]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Osteopontin antibody [EPR21139-316]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Osteopontin antibody [EPR21139-316]

  • HRP

    HRP Anti-Osteopontin antibody [EPR21139-316]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21139-316

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Assay Kits

AB269374

Human Osteopontin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Osteopontin ELISA Kit (AB269374)

  • 0
  • 0
Sample Prep & Detection Kits

AB102899

PE/Cy5.5® Conjugation Kit - Lightning-Link®

Flow Cytometry - PE/Cy5.5® Conjugation Kit - Lightning-Link® (AB102899)

  • 0
  • 0
Sample Prep & Detection Kits

AB269892

Latex Conjugation Kit - 400nm Blue

Functional Studies - Latex Conjugation Kit - 400nm Blue (AB269892)

  • 0
  • 0
Primary Antibodies

AB242851

Anti-Oncostatin M/OSM antibody [EPR19426-106] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Oncostatin M/OSM antibody [EPR19426-106] - BSA and Azide free (Detector) (AB242851)

  • 0
  • 0
Primary Antibodies

AB277997

Anti-Bone Sialoprotein antibody [EPR23243-85] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Bone Sialoprotein antibody [EPR23243-85] - BSA and Azide free (Capture) (AB277997)

  • 0
  • 0
Primary Antibodies

AB93876

Anti-Osteocalcin antibody

Western blot - Anti-Osteocalcin antibody (AB93876)

  • 3
  • 7
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

ab269411 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Osteopontin. The recommended pair for sandwich ELISA is:
Capture: ab269410, Human Osteopontin Capture Antibody (unconjugated)
Detector: ab269411, Human Osteopontin Detector Antibody (unconjugated)
The reference range value is 109.375 - 7000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.
Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
See full target information SPP1

대체 명칭 보기

BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com