Anti-OTUB2 antibody

• WB

Unknown

Western blot - Anti-OTUB2 antibody (AB74371)

All lanes:

Western blot - Anti-OTUB2 antibody (ab74371) at 1/500 dilution

Lane 1:

Extract from HepG2 cells at 5 µg

Lane 2:

Extract from Jurkat cells at 5 µg

Lane 3:

Extract from COLO 205 cells at 5 µg

Lane 4:

Extract from HT-29 cells at 5 µg

Lane 5:

Extract from 293 cells at 5 µg

Lane 6:

Extract from COLO 205 cells at 5 µg with immunising peptide

Predicted band size: 27 kDa

Observed band size: 27 kDa,34 kDa

false

• WB

CiteAb

Western blot - Anti-OTUB2 antibody (AB74371)

OTUB2 western blot using anti-OTUB2 antibody ab74371. Publication image and figure legend from Zhang, S., Zhang, M., et al., 2018, Nat Commun, PubMed 29335437.

ab74371 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab74371 please see the product overview.

Identification of USP13 as a candidate MCL1 deubiquitinase. a The quantification of MCL1 protein levels upon knockdown of each DUB in HEK293T cells. 84 siRNA pools were individually transfected into HEK293T cells, endogenous MCL1 was detected by western blot and protein bands were quantified using the Image J software. b Classification of the 84 human DUBs included in siRNA screen. c Dot plot of copy number alterations for 20 candidate MCL DUB genes in TCGA cancers. d Validation of the candidate MCL1 DUBs in HEK293T cells. Four different siRNAs targeting USP9X, OTUB2, or USP13 were transfected into HEK293T cells, and endogenous expression levels of indicated proteins were measured by western blot analysis

false