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AB250862

Anti-OVCA1 antibody [EPR15342] - BSA and Azide free

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Rabbit Recombinant Monoclonal OVCA1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

DPH2L, DPH2L1, OVCA1, DPH1, 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1, Diphthamide biosynthesis protein 1, Diphtheria toxin resistance protein 1, Ovarian cancer-associated gene 1 protein, S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1

3 이미지
Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)
  • IP

Supplier Data

Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)

This data was developed using ab185960, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from SKBR3 cell lysate immunoprecipitated using ab185960 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - C-terminal (<a href='/ko/products/primary-antibodies/ovca1-antibody-epr15342-c-terminal-ab185960'>ab185960</a>)

Predicted band size: 49 kDa

false

Western blot - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)
  • WB

Supplier Data

Western blot - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)

This data was developed using ab185960, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (<a href='/ko/products/primary-antibodies/ovca1-antibody-epr15342-c-terminal-ab185960'>ab185960</a>) at 1/10000 dilution

Lane 1:

SKBR3 cell lysate at 20 µg

Lane 2:

A375 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 49 kDa

false

Western blot - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)
  • WB

Supplier Data

Western blot - Anti-OVCA1 antibody [EPR15342] - BSA and Azide free (AB250862)

This data was developed using ab185960, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (<a href='/ko/products/primary-antibodies/ovca1-antibody-epr15342-c-terminal-ab185960'>ab185960</a>) at 1/1000 dilution

Lane 1:

SK OV 3 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15342

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB133741

Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)

  • 4
  • 15
Primary Antibodies

AB246925

Anti-DPH4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPH4 antibody (AB246925)

  • 0
  • 0
Primary Antibodies

AB125864

Anti-Phosphoribosyl pyrophosphate amidotransferase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (AB125864)

  • 0
  • 0
Primary Antibodies

AB8983

Anti-Lamin B2 antibody [LN43]

Immunohistochemistry (Frozen sections) - Anti-Lamin B2 antibody [LN43] (AB8983)

  • 3
  • 6
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

ab250862 is the carrier-free version of ab185960.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

OVCA1 also known as DPH1 or DPH2L1 is a protein encoded by a gene located in human chromosome 11p11.2. The protein weighs approximately 42 kDa. OVCA1 is involved in the early steps of diphthamide biosynthesis where it functions by interacting with its protein partners. It shows expression in various tissues with higher levels in the ovary and heart. OVCA1's role gets linked to cellular processes that impact cell growth and survival.
Biological function summary

This protein plays important roles in cellular proliferation and maintenance. OVCA1 is a part of the DPH1-DPH2 complex that facilitates the modification of eukaryotic elongation factor 2 (eEF2) through diphthamide synthesis. The process of 2-[3-carboxamido-3-(trimethylammonio)propyl]histidine diphthamide biosynthesis is essential for proper cellular protein translation. OVCA1 helps regulate the diphthamide biosynthesis pathway's first step thereby impacting the eEF2’s functionality which is important for protein synthesis and cell division.

Pathways

OVCA1's activity integrates into the cellular translation machinery where it influences the diphthamide biosynthesis pathway. This pathway is important for eEF2 modulation affecting translational control within cells. Another related pathway includes OVCA1's involvement in the cell cycle control mechanisms together with proteins like DPH2 and DPH3. By facilitating the modification of eEF2 OVCA1 effectively participates in the regulation of cellular growth impacting various growth regulatory pathways.

OVCA1 relates significantly to ovarian cancer and cardiomyopathy. Its dysfunction associates with the impaired regulation of cellular growth processes contributing to cancerous growths. OVCA1 interacts with p53 in ovarian cancer impacting tumor suppressor activities and cell cycle regulation. Additionally OVCA1 displays implications in cardiomyopathy potentially attributed to defective diphthamide biosynthesis and its downstream effects on cardiac tissue functionality. The OVCA1 and its network of associated proteins play an important part in fighting these disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2 (PubMed : 30877278). DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase (By similarity). Acts as a tumor suppressor (PubMed : 10519411).
See full target information DPH1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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