Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)

This data was developed using ab300443, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labelling Oxytocin Receptor with ab300443 at 1/50 (9.8 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in U-2 OS cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)

This data was developed using ab300443, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat-1(rat embryonic fibroblast) cells labelling Oxytocin Receptor with ab300443 at 1/50 (9.8 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in Rat-1 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• WB

Lab

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)

This data was developed using 300443, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration :

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11467983).

Negative control : skeletal muscle (PMID : 11467983).

All lanes:

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (<a href='/ko/products/primary-antibodies/oxytocin-receptor-antibody-epr24409-146-ab300443'>ab300443</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 43 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)

This data was developed using 300443, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28382233).

The blot of lane 1 was developed using a high sensitivity ECL substrate.

All lanes:

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (<a href='/ko/products/primary-antibodies/oxytocin-receptor-antibody-epr24409-146-ab300443'>ab300443</a>) at 1/1000 dilution

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Untreated C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

Lane 3:

C2C12 differentiation for 7 days, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 42 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)

This data was developed using 300443, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11467983).

Negative control : skeletal muscle (PMID : 11467983).

All lanes:

Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146] (<a href='/ko/products/primary-antibodies/oxytocin-receptor-antibody-epr24409-146-ab300443'>ab300443</a>) at 1/1000 dilution

Lane 1:

Rat uterus tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat testis tissue lysate at 20 µg

Lane 4:

Mouse uterus tissue lysate at 20 µg

Lane 5:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 6:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 42 kDa

Observed band size: 55 kDa

false

Exposure time: 3min