AB244504

Anti-p107 antibody

Name: Anti-p107 antibody
Brand: Abcam Limited
SKU: AB244504
Rating: 2 (1 reviews)

(1 리뷰)

(1 출판물)

Rabbit Polyclonal p107 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBL1 aa 550 to C-terminus.

대체 명칭 보기

Retinoblastoma-like protein 1, 107 kDa retinoblastoma-associated protein, pRb1, p107, RBL1

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Paraffin-embedded human testis tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Paraffin-embedded human colon tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Paraffin-embedded human kidney tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-p107 antibody (AB244504)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p107 antibody (AB244504)

PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma cells stained for p107 (green) using ab244504 at 4 μg/ml in ICC/IF.

Unknown

Western blot - Anti-p107 antibody (AB244504)

All lanes:

Western blot - Anti-p107 antibody (ab244504) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 121 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RBL1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P28749

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "Block in 100pc LICOR. Blocking in Milk does not work.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein p107 also known as Retinoblastoma-like protein 1 (RBL1) is a member of the retinoblastoma (RB) protein family and has a molecular weight of approximately 107 kDa. p107 is known for its role as a cell cycle regulator specifically in controlling cell cycle progression at the G1 phase. It is widely expressed in many tissues including the brain skeletal muscle and lymphoid tissues. Like other members of the RB family p107 contains an RB pocket domain important for its interactions with other proteins.

Biological function summary

Retinoblastoma-like protein 1 functions in the regulation of the cell cycle acting as an important checkpoint controller between the G1 and S phases. This protein forms a complex with E2F transcription factors which are important in controlling genes involved in cell cycle progression. The p107-E2F interaction represses transcription and inhibits the cell from proceeding to the S phase thereby ensuring proper cellular proliferation control. Moreover p107 can bind to cyclin-dependent kinases adding another layer of regulation.

Pathways

P107 plays essential roles in the RB-E2F pathway and the DNA damage response pathway. Within these pathways p107 interacts with other key proteins like p130 and pRB which together form the pocket protein family. The RB-E2F pathway controls cell cycle entry and exit while the DNA damage response pathway ensures genomic integrity. p107 acts as a mediator to pause the cell cycle during DNA damage repair collaborating with these related proteins to maintain cellular homeostasis.

P107 shows notable implications in cancer and retinoblastoma. Alterations in the expression or function of p107 can lead to unregulated cell proliferation contributing to oncogenesis. In cancer disruptions in the RB-E2F pathway often involving mutations or changes in p107 pRB or related proteins can induce tumor formation. In retinoblastoma although pRB plays a more dominant role reduced functionality of p107 can exacerbate the disease by weakening cell cycle control. Therefore p107 occupies an essential position in both safeguarding against diseases and being a potential factor in disease progression when dysregulated.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Key regulator of entry into cell division (PubMed : 17671431). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation (By similarity). Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression (By similarity). Controls histone H4 'Lys-20' trimethylation (By similarity). Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters (By similarity). Potent inhibitor of E2F-mediated trans-activation (PubMed : 8319904). May act as a tumor suppressor (PubMed : 8319904).

See full target information RBL1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Life science alliance 6: PubMed37580082

2023

Neuroprotective effects of hepatoma-derived growth factor in models of Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kerstin Voelkl,Sara Gutiérrez-Ángel,Sophie Keeling,Seda Koyuncu,Miguel da Silva Padilha,Dennis Feigenbutz,Thomas Arzberger,David Vilchez,Rüdiger Klein,Irina Dudanova

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p107 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p107 antibody (AB244504)

Immunocytochemistry/ Immunofluorescence - Anti-p107 antibody (AB244504)

Western blot - Anti-p107 antibody (AB244504)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise