AB236374

Anti-p114RhoGEF antibody [OTI6D10]

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Mouse Monoclonal p114RhoGEF antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGEF18 aa 100-250.

대체 명칭 보기

KIAA0521, ARHGEF18, Rho guanine nucleotide exchange factor 18, 114 kDa Rho-specific guanine nucleotide exchange factor, Septin-associated RhoGEF, p114-Rho-GEF, p114RhoGEF, SA-RhoGEF

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Paraffin-embedded human thyroid carcinoma tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Paraffin-embedded human breast tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Paraffin-embedded human lymph node tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

All lanes:

Western blot - Anti-p114RhoGEF antibody [OTI6D10] (ab236374) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts at 35 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) cell extracts at 35 µg

Predicted band size: 131 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI6D10

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ARHGEF18 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q6ZSZ5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

P114RhoGEF also known as ARHGEF18 is a guanine nucleotide exchange factor (GEF) specific for Rho GTPases. It functions by facilitating the exchange of GDP for GTP on RhoA a process important for activating RhoA. p114RhoGEF is approximately 114 kDa in mass. It is expressed in various tissues including vascular endothelial cells and neurons indicating a diverse range of functional roles across different systems.

Biological function summary

This protein plays an important role in the regulation of the actin cytoskeleton dynamics and cell migration. p114RhoGEF associates with other proteins to form complexes that regulate cell shape and motility. It binds with G-proteins and other signaling molecules influencing cellular responses to environmental cues such as changes in cell adhesion or polarity.

Pathways

P114RhoGEF is involved in the Rho signaling pathway which is essential for maintaining cell structure and function. Within this pathway it interacts closely with proteins like ZO-1 and Gα12/13 to control stress fiber formation and focal adhesion. It also features in pathways regulating epithelial cell junctions and endothelial barrier integrity which are critical for tissue homeostasis and vascular function.

P114RhoGEF plays a notable role in cancer progression and cardiovascular diseases. Disruptions in its function can lead to aberrant cell migration and invasion factors linked to tumor metastasis. Additionally changes in its activity might influence endothelial barrier function contributing to vascular leakiness associated with inflammatory conditions. Related proteins such as RhoA and ZO-1 also partake in these pathological processes highlighting the significance of p114RhoGEF in health and disease contexts.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells (PubMed : 22006950).

See full target information ARHGEF18

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p114RhoGEF antibody [OTI6D10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

Western blot - Anti-p114RhoGEF antibody [OTI6D10] (AB236374)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise