Anti-p150 CAF1/CAF antibody [SR3897]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p150 CAF1/CAF antibody [SR3897] (AB326699)

ICC/IF staining of p150 CAF1 in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with antibody at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [SR3897] (AB326699)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for p150 CAF1/CAF using antibody at 1/100 dilution in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-p150 CAF1/CAF antibody [SR3897] (AB326699)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : K562 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 107 kDa
Observed band size : 150 kDa

Exposure time : 5 seconds

All lanes:

Western blot - Anti-p150 CAF1/CAF antibody [SR3897] (ab326699) at 1/1000 dilution

All lanes:

K562 whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 107 kDa

Observed band size: 150 kDa

false

Exposure time: 5s

• WB

Supplier Data

Western blot - Anti-p150 CAF1/CAF antibody [SR3897] (AB326699)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : p150 CAF1 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-p150 CAF1/CAF antibody [SR3897] (ab326699) at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

p150 CAF1 Rabbit mAb knockdown Hela cell lysate at 20 µg

false