AB243734

Anti-P2X4 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal P2X4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P2RX4 aa 350-400.

대체 명칭 보기

P2X purinoceptor 4, P2X4, ATP receptor, Purinergic receptor, P2RX4

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-P2X4 antibody (AB243734)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-P2X4 antibody (AB243734)

PFA fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling P2X4 using ab243734 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis. No membranous positivity in myocytes, as expected.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Formalin-fixed, paraffin-embedded human placenta tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for P2X4 with ab243734 at a 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-P2X4 antibody (AB243734)

All lanes:

Western blot - Anti-P2X4 antibody (ab243734) at 0.4 µg/mL

All lanes:

SK-MEL-30 cell lysate

Predicted band size: 43 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human P2RX4 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

Q99571

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The P2X4 receptor also known as P2RX4 is an ATP-gated ion channel that functions as a non-selective cation channel integral to numerous physiological processes. This receptor has a molecular mass of approximately 46 kDa. You can find it expressed highly in the central nervous system specifically in microglial cells and also in various peripheral tissues such as the heart and vascular endothelial cells. The channel allows for the rapid influx of calcium sodium and potassium ions upon activation by extracellular ATP.

Biological function summary

P2X4 receptors play important roles in modulating synaptic transmission and plasticity particularly through their involvement in maintaining calcium homeostasis. These receptors do not function in isolation; they often operate as part of larger protein complexes including potential interactions with other P2X receptors such as P2X7. Through these associations P2X4 influences inflammatory responses and neuropathic pain acting as a bridge between extracellular signals and intracellular processes.

Pathways

P2X4 is important in the regulation of several signaling cascades notably the inflammatory and pain pathways. For example its interaction with the NF-kB signaling pathway highlights its contribution to inflammatory responses. P2X4 also interacts with proteins like pannexin-1 forming conduits that may amplify ATP release an important step in the propagation of inflammatory signals contributing to long-term changes in synaptic function and plasticity.

P2X4 receptor activity links to various conditions particularly neuropathic pain and heart disease. Alterations in P2X4 expression or function can contribute to the development of chronic pain conditions where enhanced P2X4 signaling is observed in microglial cells in response to nerve injury. In cardiac settings changes in P2X4 may influence heart failure with potential interactions noted with P2X7 receptors; these interactions can modulate inflammatory pathways that exacerbate cardiac dysfunction.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium (PubMed : 9016352). CTP, but not GTP or UTP, functions as a weak affinity agonist for P2RX4 (By similarity). Activated by extracellularly released ATP, it plays multiple role in immunity and central nervous system physiology (PubMed : 35165166). Plays a key role in initial steps of T-cell activation and Ca(2+) microdomain formation (By similarity). Also participates in basal T-cell activity without TCR/CD3 stimulation (By similarity). Promotes the differentiation and activation of Th17 cells via expression of retinoic acid-related orphan receptor C/RORC (PubMed : 35165166). Upon activation, drives microglia motility via the PI3K/Akt pathway (By similarity). Could also function as an ATP-gated cation channel of lysosomal membranes (By similarity).

See full target information P2RX4

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

American journal of reproductive immunology (New York, N.Y. : 1989) 94:e70132 PubMed40815040

2025

ATP/P2X4 Regulates Inflammation and Oxidative Stress in Endometriosis Through NLRP3 Inflammasome-Dependent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Wu,Yan Guo

Purinergic signalling 16:529-542 PubMed33025427

2020

The P2X7 ion channel is dispensable for energy and metabolic homeostasis of white and brown adipose tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Tian,Markus Heine,Ioannis Evangelakos,Michelle Y Jaeckstein,Nicola Schaltenberg,Tobias Stähler,Friedrich Koch-Nolte,Manju Kumari,Joerg Heeren

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P2X4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-P2X4 antibody (AB243734)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X4 antibody (AB243734)

Western blot - Anti-P2X4 antibody (AB243734)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise