JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB64960

Anti-p35 antibody

Be the first to review this product! 리뷰 제출

|

(2 제품이 사용된 논문 )

Rabbit Polyclonal CD5R1 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CDK5R1.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-p35 antibody (AB64960)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p35 antibody (AB64960)

Immunofluorescence analysis of HeLa cells, using ab64960 at 1/500 dilution. Right hand panel was also treated with immunising peptide.

Western blot - Anti-p35 antibody (AB64960)
  • WB

Unknown

Western blot - Anti-p35 antibody (AB64960)

All lanes:

Western blot - Anti-p35 antibody (ab64960) at 1/500 dilution

Lane 1:

Rat brain cell extract at 5 µg

Lane 2:

Rat brain cell extract at 5 µg with immunising peptide

Predicted band size: 34 kDa

Observed band size: 40 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CDK5R1. The exact immunogen used to generate this antibody is proprietary information.

Q15078

style
left-column

대체품

Primary Antibodies

AB235271

Anti-p35 antibody

Immunohistochemistry - Anti-p35 antibody (AB235271)

  • 0
  • 0

보완 제품

Primary Antibodies

AB40773

Anti-CDK5 antibody [EP715Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-CDK5 antibody [EP715Y] (AB40773)

  • 4
  • 7
Primary Antibodies

AB168353

Anti-CDK5RAP3 antibody [EPR11293]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (AB168353)

  • 0
  • 0
Primary Antibodies

AB134175

Anti-Cyclin D1 antibody [EPR2241] - C-terminal

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Cyclin D1 antibody [EPR2241] - C-terminal (AB134175)

  • 5
  • 15
Primary Antibodies

AB236381

Anti-Dlx1 antibody [OTI1E6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx1 antibody [OTI1E6] (AB236381)

  • 3
  • 1
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The p35 protein also known as p35 form is a regulatory subunit of the cyclin-dependent kinase 5 (Cdk5) complex. It weighs approximately 35 kDa. Expressed predominantly in the central nervous system p35 plays an important role in neural development and synaptic function. It binds to and activates Cdk5 an enzyme involved in various processes critical to neuronal function.
Biological function summary

P35 is essential for the regulation of many aspects of neural development including cell migration and axonal growth. It forms a complex with Cdk5 which then phosphorylates various substrates that are critical for these processes. The p35/Cdk5 complex influences the cytoskeletal dynamics needed for neuronal positioning and dendritic spine formation and it is significant for learning and memory.

Pathways

P35 serves in the neuronal signaling and cell cycle pathways. The Cdk5/p35 complex interfaces with pathways such as those involving the neurofilament proteins important for maintaining neuronal structure and function. In the cell cycle though atypical Cdk5/p35 can affect pathways related to cell cycle arrest highlighting its diverse functional connectivity.

P35 is significantly involved in neurodegenerative diseases like Alzheimer's and amyotrophic lateral sclerosis (ALS). In Alzheimer’s abnormal p35 cleavage to p25 by enzymes like calpain results in hyperactivity of Cdk5 leading to neuronal damage. The similarity in pathways also connects p35 to other neuronal proteins such as tau where misregulation can result in tauopathy a feature observed in several neurodegenerative disorders.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein : phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-BMAL1 heterodimer in association with altered stability and subcellular distribution.
See full target information CDK5R1

대체 명칭 보기

CDK5R, NCK5A, CDK5R1, Cyclin-dependent kinase 5 activator 1, CDK5 activator 1, Cyclin-dependent kinase 5 regulatory subunit 1, TPKII regulatory subunit
style
left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oxidative medicine and cellular longevity 2020:2616871 PubMed33456665

2020

Involvement of the miR-137-3p/CAPN-2 Interaction in Ischemia-Reperfusion-Induced Neuronal Apoptosis through Modulation of p35 Cleavage and Subsequent Caspase-8 Overactivation.

Applications

Unspecified application

Species

Unspecified reactive species

He Wang,Qian Yu,Zai-Li Zhang,Hong Ma,Xiao-Qian Li

Oncotarget 7:36510-36528 PubMed27145370

2016

CDK5 promotes renal tubulointerstitial fibrosis in diabetic nephropathy via ERK1/2/PPARγ pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Bai,Xiaoyan Hou,Jianwei Tian,Jian Geng,Xiao Li
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com