AB211527

Anti-P4HA2 antibody [CL0351]

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Mouse Monoclonal P4HA2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P4HA2 aa 1-200.

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue labeling P4HA2 with ab211527 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling P4HA2 with ab211527 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling P4HA2 with ab211527 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

Immunohistochemical analysis of paraffin-embedded Human lung cancer (adenocarcinoma) tissue labeling P4HA2 with ab211527 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling P4HA2 with ab211527 at 1/200 dilution.

Supplier Data

Western blot - Anti-P4HA2 antibody [CL0351] (AB211527)

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

All lanes:

Western blot - Anti-P4HA2 antibody [CL0351] (ab211527)

Lane 1:

U-251 cells, transfected with control siRNA

Lane 2:

U-251 cells, transfected with target specific siRNA probe #1

Lane 3:

U-251 cells, transfected with target specific siRNA probe #2

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-P4HA2 antibody [CL0351] (AB211527)

All lanes:

Western blot - Anti-P4HA2 antibody [CL0351] (ab211527) at 1/500 dilution

All lanes:

U251 MG cell line lysate

Predicted band size: 61 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0351

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human P4HA2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O15460

Epitope

Binds to an epitope located within the peptide sequence NTDWPALEDLVLQDS (94-108aa) as determined by overlapping synthetic peptides.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

P4HA2 also known as Prolyl 4-hydroxylase subunit alpha-2 is an enzyme with an approximate mass of 60 kDa. It plays a role in catalyzing the hydroxylation of proline residues in collagen strands which is essential for the proper formation of the collagen triple helix structure. P4HA2 can be found most abundantly in tissues that require strong extracellular matrices such as skin tendons and cartilage. Its expression in these tissues suggests a critical role in maintaining structural integrity.

Biological function summary

Prolyl 4-hydroxylase functions within a larger enzyme complex by working alongside other subunits to ensure efficient collagen biosynthesis. This complex modifies proline residues on collagen peptides ensuring structural stability and mechanical strength of the extracellular matrix. By contributing to collagen stability P4HA2 supports cellular functions related to tissue maintenance and repair. Its activity is important in physiological environments where collagen is abundant emphasizing its role in vertebrate development and organism physiology.

Pathways

Collagen biosynthesis and modification serve as significant pathways involving P4HA2. This enzyme contributes to pathways related to the post-translational modification of collagen which is essential for extracellular matrix organization and cellular function. P4HA2 interacts with proteins such as collagen type I and type III playing an important role in the maturation of collagen fibers and influencing tissue resilience and elasticity. Such interactions highlight its involvement in fundamental biological processes that sustain organismal structure and function.

P4HA2 has associations with fibrosis and certain cancers. During fibrotic progression dysregulation of collagen production often involves elevated P4HA2 levels driving excessive extracellular matrix deposition and tissue stiffening. This condition can connect P4HA2 to fibrosis-related proteins such as TGF-beta which regulate extracellular matrix production. In cancer P4HA2 may contribute to tumor microenvironment changes influencing tumor growth and metastasis through interactions with proteins like MMPs (matrix metalloproteinases). Understanding these connections not only helps in identifying potential therapeutic targets but also in elucidating the pathophysiology of collagen-related diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

See full target information P4HA2

대체 명칭 보기

UNQ290/PRO330, P4HA2, Prolyl 4-hydroxylase subunit alpha-2, 4-PH alpha-2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in oncology 15:1581860 PubMed40406250

2025

Integrated analysis identifies P4HA2 as a key regulator of STAT1-mediated colorectal cancer progression and a potential biomarker for precision therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Qianshi Zhang,Yinan Zhang,Zhiwei Sun,Huanle Wang,Guohang Dai,Yue Meng,Shasha Shi,Shuangyi Ren

International journal of medical sciences 19:1796-1805 PubMed36313222

2022

The Inhibition of Adipose-Derived Stem Cells on the Invasion of Keloid Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Jiong Zhou,Ji-Yang Shen,Li-En Tao,Huan Chen

British journal of cancer 119:1518-1526 PubMed30410060

2018

Prolyl-4-hydroxylase Α subunit 2 (P4HA2) expression is a predictor of poor outcome in breast ductal carcinoma in situ (DCIS).

Applications

Unspecified application

Species

Unspecified reactive species

Michael S Toss,Islam M Miligy,Kylie L Gorringe,Abdulbaqi AlKawaz,Hazem Khout,Ian O Ellis,Andrew R Green,Emad A Rakha

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P4HA2 antibody [CL0351]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody [CL0351] (AB211527)

Western blot - Anti-P4HA2 antibody [CL0351] (AB211527)

Western blot - Anti-P4HA2 antibody [CL0351] (AB211527)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise