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AB239880

Anti-p53 (phospho S46) antibody [EP42Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal P53 phospho S46 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
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Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S46) antibody [EP42Y] - BSA and Azide free (AB239880)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S46) antibody [EP42Y] - BSA and Azide free (AB239880)

Immunocytochemistry/immunofluorescence staining of 4% PFA fixed; 0.1% Triton-X permeabilized A431 (human epidermoid carcinoma) cells with ab76242 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/500) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000).

Confocal image showed increased nuclear staining after ETP (etoposide; 25 μM, 5h) treatment on A431 cells. The LP treatment decreased the increased nuclear staining caused by ETP.

ab179477 (1/500) was used as a Pan control for ab76242. The results showed nuclear staining on untreated, ETP and ETP+LP treated Hela cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76242).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S46) antibody [EP42Y] - BSA and Azide free (AB239880)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S46) antibody [EP42Y] - BSA and Azide free (AB239880)

Immunohistochemical analysis of human ovarian adenocarcinoma using ab76242 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76242).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP42Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB183544

Anti-p53 (acetyl K370) antibody [EPR17496]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunoprecipitation - Anti-p53 (acetyl K370) antibody [EPR17496] (AB183544)

  • 4
  • 1
Primary Antibodies

AB32389

Anti-p53 antibody [E26]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [E26] (AB32389)

  • 0
  • 0
Primary Antibodies

AB75754

Anti-p53 (acetyl K382) antibody [EPR358(2)]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-p53 (acetyl K382) antibody [EPR358(2)] (AB75754)

  • 5
  • 4
Primary Antibodies

AB33889

Anti-p53 (phospho S392) antibody [EP155Y]

RabMAb|Lab Essentials|20ul selling size|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S392) antibody [EP155Y] (AB33889)

  • 3
  • 1
Primary Antibodies

AB179477

Anti-p53 antibody [EPR17343]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [EPR17343] (AB179477)

  • 3
  • 2
Primary Antibodies

AB223868

Anti-p53 (phospho S15) antibody [EPR64(N)] - ChIP Grade

RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S15) antibody [EPR64(N)] - ChIP Grade (AB223868)

  • 4
  • 1

보완 제품

Sample Prep & Detection Kits

AB102921

Streptavidin Conjugation Kit - Lightning-Link®

Conjugation - Streptavidin Conjugation Kit - Lightning-Link® (AB102921)

  • 0
  • 0
Sample Prep & Detection Kits

AB270705

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (AB270705)

  • 0
  • 0
Primary Antibodies

AB223868

Anti-p53 (phospho S15) antibody [EPR64(N)] - ChIP Grade

RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-p53 (phospho S15) antibody [EPR64(N)] - ChIP Grade (AB223868)

  • 4
  • 1
Primary Antibodies

AB109520

Anti-p21 antibody [EPR362]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-p21 antibody [EPR362] (AB109520)

  • 5
  • 17
Primary Antibodies

AB1101

Anti-p53 antibody [DO-1]

BOND RX™ Validated|KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [DO-1] (AB1101)

  • 4
  • 14
Primary Antibodies

AB33889

Anti-p53 (phospho S392) antibody [EP155Y]

RabMAb|Lab Essentials|20ul selling size|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S392) antibody [EP155Y] (AB33889)

  • 3
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

ab239880 is the carrier-free version of ab76242.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 35618207, PubMed : 36634798, PubMed : 38653238, PubMed : 9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17189187, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 38653238, PubMed : 9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed : 12524540, PubMed : 17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed : 12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed : 12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed : 24051492).
See full target information TP53 phospho S46

대체 명칭 보기

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell metabolism 36:1764-1778.e9 PubMed38889724

2024

Short-term cold exposure induces persistent epigenomic memory in brown fat.

Applications

Unspecified application

Species

Unspecified reactive species

Shin-Ichi Inoue,Matthew J Emmett,Hee-Woong Lim,Mohit Midha,Hannah J Richter,Isaac J Celwyn,Rashid Mehmood,Maria Chondronikola,Samuel Klein,Amy K Hauck,Mitchell A Lazar
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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