AB8105

Anti-p53R2 antibody

Name: Anti-p53R2 antibody
Brand: Abcam Limited
SKU: AB8105
Rating: 5 (2 reviews)

(2 리뷰들)

(30 제품이 사용된 논문 )

Rabbit Polyclonal p53R2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human RRM2B aa 1-50.

1 이미지

Supplier Data

Western blot - Anti-p53R2 antibody (AB8105)

All lanes:

Western blot - Anti-p53R2 antibody (ab8105) at 1 µg/mL

Lane 1:

3T3 cell lysate with absence of blocking peptide

Lane 2:

3T3 cell lysate with presence of blocking peptide

Predicted band size: 40 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RRM2B aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q7LG56

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

P53R2 also known as RRM2B is a ribonucleotide reductase subunit with a molecular mass of 41 kDa. This protein is primarily expressed in the cytoplasm and mitochondria of various cell types including liver and kidney tissues. Mechanically p53R2 functions by reducing ribonucleotides into deoxyribonucleotides which are necessary for DNA synthesis and repair. This process is important for maintaining genomic stability especially under conditions of cellular stress or damage.

Biological function summary

P53R2 plays a role in the synthesis of deoxyribonucleotide triphosphates (dNTPs) for DNA repair. It forms part of a ribonucleotide reductase complex working with other subunits to ensure cells have sufficient dNTP pools for DNA replication and repair. The presence of p53R2 is integral for DNA damage response pathways where it gets activated to supply dNTPs during periods when the p53 tumor suppressor pathway senses DNA damage and activates DNA repair processes.

Pathways

P53R2 is involved in DNA damage response and cell cycle regulation. It interacts with the tumor suppressor protein p53 especially during instances of genotoxic stress facilitating DNA repair and cell cycle arrest to prevent propagation of damaged DNA. Additionally p53R2 relates to proteins such as p21 which are components of the p53 pathway influencing cell cycle arrest and allowing time for DNA repair before cell division resumes.

Researchers link p53R2 to cancer and mitochondrial depletion syndrome. In cancer mutations or dysregulated expression of p53R2 can impair DNA repair leading to genomic instability and tumorigenesis. The protein associates with p53 in these processes and its dysfunction may contribute to cancer progression. Furthermore mutations in p53R2 have implications in mitochondrial depletion syndrome where reduced functionality can affect mitochondrial DNA maintenance and cellular energy metabolism.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.

See full target information RRM2B

대체 명칭 보기

P53R2, RRM2B, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein, p53R2

style

left-column

제품이 사용된 논문 (30)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nucleic acids research 52:12351-12377 PubMed39315696

2024

p53-dependent crosstalk between DNA replication integrity and redox metabolism mediated through a NRF2-PARP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Gamal Ahmed Elfar,Obed Aning,Tsz Wai Ngai,Pearlyn Yeo,Joel Wai Kit Chan,Shang Hong Sim,Leonard Goh,Ju Yuan,Cheryl Zi Jin Phua,Joanna Zhen Zhen Yeo,Shi Ya Mak,Brian Kim Poh Goh,Pierce Kah-Hoe Chow,Wai Leong Tam,Ying Swan Ho,Chit Fang Cheok

Cancers 15: PubMed37444452

2023

Radiation-Induced Cellular Senescence Reduces Susceptibility of Glioblastoma Cells to Oncolytic Vaccinia Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Quinn T Storozynsky,Xuefei Han,Shae Komant,Kate C Agopsowicz,Kyle G Potts,Armin M Gamper,Roseline Godbout,David H Evans,Mary M Hitt

EMBO molecular medicine 12:e10419 PubMed31950591

2020

Ribonucleotide reductase inhibitors suppress SAMHD1 ara-CTPase activity enhancing cytarabine efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Sean G Rudd,Nikolaos Tsesmetzis,Kumar Sanjiv,Cynthia Bj Paulin,Lakshmi Sandhow,Juliane Kutzner,Ida Hed Myrberg,Sarah S Bunten,Hanna Axelsson,Si Min Zhang,Azita Rasti,Petri Mäkelä,Si'Ana A Coggins,Sijia Tao,Sharda Suman,Rui M Branca,Georgios Mermelekas,Elisée Wiita,Sun Lee,Julian Walfridsson,Raymond F Schinazi,Baek Kim,Janne Lehtiö,Georgios Z Rassidakis,Katja Pokrovskaja Tamm,Ulrika Warpman-Berglund,Mats Heyman,Dan Grandér,Sören Lehmann,Thomas Lundbäck,Hong Qian,Jan-Inge Henter,Torsten Schaller,Thomas Helleday,Nikolas Herold

OncoTargets and therapy 12:8367-8378 PubMed31632084

2019

miR-942 promotes proliferation and metastasis of hepatocellular carcinoma cells by inhibiting RRM2B.

Applications

Unspecified application

Species

Unspecified reactive species

Qifan Zhang,Bili Zhu,Jianping Qian,Kai Wang,Jie Zhou

Frontiers in oncology 9:956 PubMed31637211

2019

Integrative Analysis Reveals Across-Cancer Expression Patterns and Clinical Relevance of Ribonucleotide Reductase in Human Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Yongfeng Ding,Tingting Zhong,Min Wang,Xueping Xiang,Guoping Ren,Zhongjuan Jia,Qinghui Lin,Qian Liu,Jingwen Dong,Linrong Li,Xiawei Li,Haiping Jiang,Lijun Zhu,Haoran Li,Dejun Shen,Lisong Teng,Chen Li,Jimin Shao

Cancer management and research 10:3505-3511 PubMed30271199

2018

CD8+ tumor-infiltrating lymphocytes as a novel prognostic biomarker in lung sarcomatoid carcinoma, a rare subtype of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiewei Chen,Qingmei He,Jun Liu,Yongbo Xiao,Canhua Xiao,Keming Chen,Dan Xie,Xinke Zhang

Nature chemical biology 14:943-954 PubMed30150681

2018

Nuclear RNR-α antagonizes cell proliferation by directly inhibiting ZRANB3.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Fu,Marcus J C Long,Somsinee Wisitpitthaya,Huma Inayat,Timothy M Pierpont,Islam M Elsaid,Jordana C Bloom,Joaquin Ortega,Robert S Weiss,Yimon Aye

BMC cancer 17:855 PubMed29246119

2017

Overexpression of p53R2 is associated with poor prognosis in lung sarcomatoid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiewei Chen,Yongbo Xiao,Xiaoyan Cai,Jun Liu,Keming Chen,Xinke Zhang

Oncotarget 8:101572-101590 PubMed29254187

2017

Transcriptomic pathway analysis of urokinase receptor silenced breast cancer cells: a microarray study.

Applications

Unspecified application

Species

Unspecified reactive species

Pavan B Narayanaswamy,Tapan K Baral,Hermann Haller,Inna Dumler,Kshitish Acharya,Yulia Kiyan

Cell cycle (Georgetown, Tex.) 16:1673-1682 PubMed28841361

2017

p53R2 overexpression in cervical cancer promotes AKT signaling and EMT, and is correlated with tumor progression, metastasis and poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Jiang,Rui Xu,Xiao-Xing Li,Yan-Yan Wang,Wen-Qian Liang,Ju-Deng Zeng,Shan-Shan Zhang,Xiao-Yi Xu,Yang Yang,Mei-Yin Zhang,Hui-Yun Wang,X F Steven Zheng

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p53R2 antibody

Western blot - Anti-p53R2 antibody (AB8105)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (30)

Product promise