AB92581

Anti-p63 (phospho S395) antibody

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Rabbit Polyclonal P63 phospho S395 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TP63 pS395.

1 이미지

Western blot - Anti-p63 (phospho S395) antibody (AB92581)

WB

Unknown

Western blot - Anti-p63 (phospho S395) antibody (AB92581)

All lanes:

Western blot - Anti-p63 (phospho S395) antibody (ab92581) at 1/500 dilution

Lane 1:

Extracts from HepG2 cells treated with nocodazole (1 µg/ml,18 hours) at 30 µg

Lane 2:

Extracts from A549cells treated with nocodazole (1 µg/ml,18 hours) at 30 µg

Lane 3:

Extracts from LOVO cells treated with nocodazole (1 µg/ml,18 hours) at 30 µg

Lane 4:

Extracts from HepG2 cells treated with nocodazole (1 µg/ml,18 hours) at 30 µg with immunizing peptide

Predicted band size: 77 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

Immunogen

Synthetic Peptide within Human TP63 pS395. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB53039

Anti-p63 antibody

Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody (AB53039)

5
10

Primary Antibodies

AB735

Anti-p63 antibody [4A4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [4A4] (AB735)

5
12

Primary Antibodies

AB246728

Alexa Fluor® 647 Anti-p63 antibody [EPR5701]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-p63 antibody [EPR5701] (AB246728)

0
0

Primary Antibodies

AB202861

Biotin Anti-p63 antibody [EPR5701]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-p63 antibody [EPR5701] (AB202861)

0
0

Primary Antibodies

AB202357

HRP Anti-p63 antibody [EPR5701]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-p63 antibody [EPR5701] (AB202357)

0
0

Primary Antibodies

AB124762

Anti-p63 antibody [EPR5701]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] (AB124762)

4
13

Primary Antibodies

AB32353

Anti-p63 antibody [Y289]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-p63 antibody [Y289] (AB32353)

5
1

Primary Antibodies

AB214790

Anti-p63 antibody [EPR5701] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-p63 antibody [EPR5701] - BSA and Azide free (AB214790)

0
0

보완 제품

Primary Antibodies

AB124762

Anti-p63 antibody [EPR5701]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] (AB124762)

4
13

Primary Antibodies

AB69565

Anti-HIPK4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HIPK4 antibody (AB69565)

0
0

Primary Antibodies

AB52635

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

5
11

Primary Antibodies

AB137691

Anti-p40 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 antibody (AB137691)

0
0

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The p63 protein also known as TP63 or KET is a transcription factor that plays a role in the regulation of cell cycle and apoptosis. With a molecular mass of approximately 77 kDa it expresses predominantly in epithelial tissues such as those in the skin breast and prostate. The expression pattern of p63 makes it a useful marker in developmental biology and cancer diagnosis. Antibodies like '4A4' specifically recognize p63 in various assays including immunohistochemistry.

Biological function summary

P63 participates in the control of epithelial cell differentiation and proliferation. It functions as a member of the p53 family which includes p53 and p73. This family forms an important part of the complex regulatory network that maintains genomic stability. Research using methods like p63 staining in immunohistochemistry highlights its critical role in stratified epithelia. The gene encodes several isoforms with different biological activities impacting processes such as cellular senescence and development.

Pathways

P63 integrates into various signaling pathways that influence cellular growth and adherence. It notably impacts the Notch and Wnt pathways both of which are critical for developmental processes and cancer progression. Through these pathways p63 interacts with proteins like β-catenin and Notch receptors facilitating cross-talk that influences cell fate decisions. Its function relates closely to other transcription factors forming feedback loops that reinforce its regulatory impact.

P63 shows strong associations with ectodermal dysplasia and different types of cancers like squamous cell carcinoma. Mutations and altered expressions of p63 contribute to these pathologies by disrupting normal cell proliferation and differentiation. The protein interacts closely with p73 in cancer suggesting a compensatory role when p63 function is compromised. Understanding these connections can guide the development of targeted therapies and improve diagnostic accuracy.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.

See full target information TP63 pS395

대체 명칭 보기

KET, P63, P73H, P73L, TP73L, TP63, Tumor protein 63, p63, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor KET, Transformation-related protein 63, Tumor protein p73-like, p40, p51, CUSP, p73L

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in bioengineering and biotechnology 13:1555983 PubMed40599407

2025

Distribution and metabolism of iPSC-MSCs in the joint cavity of an osteoarthritis rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyang Yuan,Xulei Wang,Tianxi Du,Feng Zhang,Gang Cheng,Kang Wang,Haochen Xu,Pingting Yang,Yan Chang,Wei Wei,Peng He,Shangxue Yan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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