AB216495

Anti-p75 NGF Receptor antibody

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Rabbit Polyclonal p75 NGF Receptor antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Rat Ngfr aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

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Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (AB216495)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (AB216495)

Immunofluorescent analysis of HepG2 cells labeling p75 NGF Receptor with ab216495 at 1/500 dilution in PBS (incubated for two hours at 37°C), followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB216495)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB216495)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat liver tissue labeling p75 NGF Receptor with ab216495 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, PE conjugated at 1/200 dilution for 40 minutes at 37°C.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB216495)

Flow Cytometry - Anti-p75 NGF Receptor antibody (AB216495)

Flow Cyt

Unknown

Flow Cytometry - Anti-p75 NGF Receptor antibody (AB216495)

Flow Cytometry of Mouse Spleen with ab217628 at 1/100

Supplier Data

Western blot - Anti-p75 NGF Receptor antibody (AB216495)

All lanes:

Western blot - Anti-p75 NGF Receptor antibody (ab216495) at 1/300 dilution

All lanes:

Mouse brain lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

Predicted band size: 45 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Rat Ngfr aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P07174

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "(or 1/50 - 1/200 if using a fluorescent secondary antibody).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.

Biological function summary

The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.

Pathways

The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.

P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed : 24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed : 26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed : 23785138). Together with BFAR negatively regulates NF-kappa-B and JNK-related signaling pathways (PubMed : 22566094).

See full target information NGFR

대체 명칭 보기

CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Cellular and molecular life sciences : CMLS 82:330 PubMed40886189

2025

Melatonin attenuates intervertebral disc degeneration by restoring mitochondrial homeostasis through PGC-1α signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Lan,Wenhan Yang,Bin Yan,Weizhuang Guo,Yuantao Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-p75 NGF Receptor antibody

Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (AB216495)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB216495)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody (AB216495)

Flow Cytometry - Anti-p75 NGF Receptor antibody (AB216495)

Western blot - Anti-p75 NGF Receptor antibody (AB216495)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise