Anti-PADI4 / PAD4 antibody

• WB

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Western blot - Anti-PADI4 / PAD4 antibody (AB50332)

All lanes:

Western blot - Anti-PADI4 / PAD4 antibody (ab50332) at 1/1000 dilution

Lane 1:

293-T cells

Lane 2:

293-T cells transfected with PADI4 cDNA

Lane 3:

HL60 cells

Lane 4:

HL60 cells induced with 1.25% DMSO for three days

Secondary

All lanes:

Goat Anti-Rabbit IgG

Predicted band size: 74 kDa

Observed band size: ~62 kDa

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• WB

CiteAb

Western blot - Anti-PADI4 / PAD4 antibody (AB50332)

PADI4 / PAD4 western blot using anti-PADI4 / PAD4 antibody ab50332. Publication image and figure legend from Sharma, P., Azebi, S., et al., 2012, PLoS Genet, PubMed 23028349.

ab50332 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab50332 please see the product overview.

An antibody specific for histone H3cit8K9me3 reveals increased levels of this double modification catalyzed by PADI4.(A) Epitope used to generate the anti-H3cit8K9me3 antibody. The antibody was raised against a peptide mimicking histone H3 citrullinated at position 8 and tri-methylated on K9. (B) Dot blot highlighting the specificity of the anti-H3cit8K9me3, performed with 1, 0.2, and 0.04 µg of H3 peptides bearing the indicated post-translational modifications. (C) Specificity of the anti-H3cit8K9me3 antibody test by competition with H3 peptides. MCF7 cells were extracted, fixed and incubated with the indicated peptides along with anti-H3cit8K9me3 antibodies and DAPI. Scale bar 5 µm. (D) Similar staining with anti-H3cit8K9me3 and anti-H3cit(2,8,17) antibodies. Indirect immunofluorescent staining of MCF7 cells with DAPI to visualize the DNA and either anti-H3cit8K9me3 (b1–b2) or anti-H3cit(2,8,17) (b3–b4). (E) HEK293 cells that stably integrate a ponasterone A-inducible construct with either no insert (NI) or an insert coding for wild-type (WT), hyperactive (Mut+), or hypoactive (Mut−) PADI4 were cultured without or with ionophore (A23187). Total protein extracts were immunoblotted with the indicated antibodies. Signals were quantified with the Image J software (NI without treatment set to 1).

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