AB40808

Anti-PAK3 antibody [EP797Y]

RabMAb
Recombinant
각 태그의 뜻

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(4 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal PAK3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] (AB40808)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] (AB40808)

Immunohistochemical analysis of paraffin-embedded human brain tissue labeling PAK3 with ab40808 at 1/50 dilution (3.98 μg/ml).

Positive staining on human brain.

The section was incubated with ab40808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] (AB40808)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling PAK3 with ab40808 at 1/50 dilution (3.98 μg/ml).

Positive staining on mouse brain.

The section was incubated with ab40808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] (AB40808)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling PAK3 with ab40808 at 1/50 dilution (3.98 μg/ml).

Positive staining on rat brain.

The section was incubated with ab40808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

Lab

Western blot - Anti-PAK3 antibody [EP797Y] (AB40808)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

Negative samples : liver and kidney (PMID : 9731525).

All lanes:

Western blot - Anti-PAK3 antibody [EP797Y] (ab40808) at 1/2000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse kidney tissue lysate at 20 µg

Lane 7:

Rat brain tissue lysate at 20 µg

Lane 8:

Rat kidney tissue lysate at 20 µg

Lane 9:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 65 kDa,36 kDa

false

Exposure time: 180s

Unknown

Western blot - Anti-PAK3 antibody [EP797Y] (AB40808)

All lanes:

Western blot - Anti-PAK3 antibody [EP797Y] (ab40808) at 1/50000 dilution

All lanes:

fetal brain lysate at 10 µg

Predicted band size: 62 kDa

Observed band size: 65 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP797Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC) (By similarity).

See full target information PAK3

대체 명칭 보기

OPHN3, PAK3, Serine/threonine-protein kinase PAK 3, Beta-PAK, Oligophrenin-3, p21-activated kinase 3, PAK-3

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of Cancer 13:153-161 PubMed34976179

2022

PAK3 promotes the metastasis of hepatocellular carcinoma by regulating EMT process.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Gao,Mengya Zhong,Zhijian Ye,Zhengxin Wu,Yubo Xiong,Jinsong Ma,Huiyu Chen,Yuekun Zhu,Yan Yang,Yongxiang Zhao,Zhiyong Zhang

Cell biology international 44:2284-2292 PubMed32749739

2020

LncRNA TTN-AS1/miR-134-5p/PAK3 axis regulates the radiosensitivity of human large intestine cancer cells through the P21 pathway and AKT/GSK-3β/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenkui Zuo,Shuling Ji,Lulu He,Yage Zhang,Zining Peng,Jiarui Han

Brain pathology (Zurich, Switzerland) 30:319-331 PubMed31410926

2019

Synaptic actin stabilization protein loss in Down syndrome and Alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Julie C Lauterborn,Conor D Cox,See Wing Chan,Peter W Vanderklish,Gary Lynch,Christine M Gall

Nature methods 13:770-6 PubMed27479327

2016

Revealing disease-associated pathways by network integration of untargeted metabolomics.

Applications

Unspecified application

Species

Unspecified reactive species

Leila Pirhaji,Pamela Milani,Mathias Leidl,Timothy Curran,Julian Avila-Pacheco,Clary B Clish,Forest M White,Alan Saghatelian,Ernest Fraenkel

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAK3 antibody [EP797Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] (AB40808)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] (AB40808)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] (AB40808)

Western blot - Anti-PAK3 antibody [EP797Y] (AB40808)

Western blot - Anti-PAK3 antibody [EP797Y] (AB40808)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise