AB316335

Anti-pan NOS antibody [RM1126] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(0 출판물)

Rabbit Recombinant Multiclonal eNOS antibody. Carrier free. Suitable for Dot, IHC-P and reacts with Recombinant full length protein - Human, Human, Transfected cell line - Human samples.

9 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

This data was developed using ab316334, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human liver. The section was incubated with ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

This data was developed using ab316334, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T transfected with a human nNOS expression vector containing a Myc-His tag, (B) HEK-293T transfected with a human iNOS expression vector containing a Myc-His tag, and (C) HEK-293T transfected with a human eNOS expression vector containing a Myc-His tag, no staining on (D) HEK-293T transfected with empty vector containing a Myc-His tag tissues labeling pan NOS with ab316334 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on (A) HEK-293T transfected with a human nNOS expression vector containing a Myc-His tag, (B) HEK-293T transfected with a human iNOS expression vector containing a Myc-His tag, and (C) HEK-293T transfected with a human eNOS expression vector containing a Myc-His tag, no staining on (D) HEK-293T transfected with empty vector containing a Myc-His tag. The section was incubated with ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

This data was developed using ab316334, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on endothelial cells in human cervical squamous carcinoma. The section was incubated with ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

This data was developed using ab316334, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on endothelial cells in human spleen. The section was incubated with ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

This data was developed using ab316334, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human thalamus tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human thalamus. The section was incubated with ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

This data was developed using ab316334, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human cerebellum. The section was incubated with ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

This data was developed using ab316334, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human placenta. The section was incubated with ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

This data was developed using ab316334, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling pan NOS with ab316334 at 1/200 (2.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human lung. The section was incubated with ab316334 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Dot Blot - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

This data was developed using ab316334, the same antibody clone in a different buffer formulation.

Dot blot analysis of pan NOS using ab316334 at 1 : 1000 (0.503 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 20,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) as total protein control staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-pan NOS antibody [RM1126] (<a href='/ko/products/primary-antibodies/pan-nos-antibody-rm1126-ab316334'>ab316334</a>) at 1/1000 dilution

Lane 1:

C-Myc/DDK tagged full length human nNOS recombinant protein

Lane 2:

C-Myc/DDK tagged full length human iNOS recombinant protein

Lane 3:

C-Myc/DDK tagged full length human eNOS recombinant protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 1s

주요 정보

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1126

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Dot

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell line - Human": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

제품 세부 정보

ab316335 is the carrier-free version of ab316334.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Nitric Oxide Synthases (NOS) are enzymes responsible for the production of nitric oxide (NO) from L-arginine. NOS proteins exist in three main isoforms: neuronal NOS (nNOS or NOS1) inducible NOS (iNOS or NOS2) and endothelial NOS (eNOS or NOS3). They range in molecular mass between 130 and 160 kDa. Pan NOS refers to any antibody or inhibitor that targets all three NOS isoforms. These enzymes are expressed in various tissues with nNOS mainly in neural tissue iNOS in immune cells and eNOS in endothelial cells. Each isoform plays a unique role in its respective location.

Biological function summary

Nitric oxide acts as a signaling molecule with diverse functions within the body. It does not bind into large permanent complexes but interacts transiently with various proteins to relay signals. In the nervous system nNOS-generated NO modulates neurotransmission and contributes to synaptic plasticity. In blood vessels eNOS-derived NO plays an important role in vasodilation and blood pressure regulation. iNOS often gets activated during immune responses producing larger amounts of NO to fight pathogens.

Pathways

Nitric oxide produced by NOS enzymes participates in significant signaling cascades. The L-arginine-NO-cGMP pathway is one of the primary routes involving cyclic guanosine monophosphate (cGMP) as a secondary messenger. The NO-cGMP pathway interacts with proteins like soluble guanylyl cyclase which then modulates various physiological responses like vasodilation and neurotransmission. NOS activity also interplays with oxidative stress pathways interacting with reactive oxygen species (ROS) and affecting cellular responses like inflammation and apoptosis.

Aberrations in NOS activity relate to cardiovascular disorders and neurodegenerative diseases. For instance dysregulated eNOS activity can contribute to hypertension due to impaired vasodilation. In neurodegenerative conditions such as Alzheimer's disease abnormal nNOS activity may exacerbate neuronal damage through excessive NO production. Furthermore iNOS expression links to inflammatory conditions where excess NO might damage tissues. NOS activity can also intersect with proteins like amyloid-beta in Alzheimer's amplifying neurodegenerative processes.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed : 1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

See full target information NOS3

Additional targets

Nitric oxide synthase, brain,NOS2,Nos2

대체 명칭 보기

Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3, NOS2A, NOS2, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS, Inosl, Nos2, Inducible NO synthase, Macrophage NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, Inducible NOS, iNOS, MAC-NOS, Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS, NOS1

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-pan NOS antibody [RM1126] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

Dot Blot - Anti-pan NOS antibody [RM1126] - BSA and Azide free (AB316335)

관련 conjugated 항체와 다양한 조성의 항체 (6)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

대체 명칭 보기

Product promise