AB6176

Anti-pan Synuclein antibody

Name: Anti-pan Synuclein antibody
Brand: Abcam Limited
SKU: AB6176
Rating: 4 (4 reviews)

(4 리뷰들)

(18 제품이 사용된 논문 )

Rabbit Polyclonal Alpha-synuclein antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Rat, Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA aa 1-50.

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (AB6176)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (AB6176)

ICC/IF image of ab6176 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6176, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat

Applications

IHC-P, ICC/IF, IHC-Fr, WB

applications

Immunogen

Synthetic Peptide within Human SNCA aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P37840

Specificity

This antibody recognises all 3 forms of the synucleins.

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Reactivity 정보

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특성 및 보관 정보

제형

Liquid

Purity

Whole antiserum

보관 버퍼

pH: 7 - 7.4

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Add glycerol to a final volume of 50% for extra stability and aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pan Synuclein also known as alpha beta and gamma synucleins are small proteins with molecular masses around 14-19 kDa. These proteins are expressed largely in the central nervous system and are also present in other tissues such as the peripheral nervous system and certain types of muscles. They share structural similarities and comprise a family often involved with neurons where they play roles in various cellular processes.

Biological function summary

Synucleins participate in the regulation of synaptic function and plasticity influencing neurotransmitter release and membrane dynamics. They do not form a specific complex but can interact with lipid membranes and various cellular proteins affecting vesicular transport processes. These proteins hold influence over essential cellular functions that depend on synaptic activity and efficiency.

Pathways

Synucleins involve themselves in important neuronal pathways such as those controlling dopamine neurotransmission and regulation of mitochondrial functions. They interact with key proteins like parkin and ubiquitin which participate in protein degradation pathways. Synucleins' roles in these pathways highlight their impact on both synaptic transmission and energy metabolism within neurons.

Synucleins have strong connections to neurodegenerative disorders like Parkinson's disease and dementia with Lewy bodies. In these disorders alpha-synuclein in particular forms aggregates known as Lewy bodies contributing to neuronal dysfunction. These aggregates interact with proteins such as tau and amyloid-beta further linking synucleins to the pathology of these disorders. Understanding these interactions provides insights into potential targets for therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA

대체 명칭 보기

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

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제품이 사용된 논문 (18)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 300:107419 PubMed38815862

2024

Neuronal activity promotes secretory autophagy for the extracellular release of α-synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshitsugu Nakamura,Taiki Sawai,Kensuke Kakiuchi,Shigeki Arawaka

Frontiers in cell and developmental biology 9:774650 PubMed34901020

2021

Synuclein Regulates Synaptic Vesicle Clustering and Docking at a Vertebrate Synapse.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlyn E Fouke,M Elizabeth Wegman,Sarah A Weber,Emily B Brady,Cristina Román-Vendrell,Jennifer R Morgan

Neurobiology of disease 151:105256 PubMed33429042

2021

Doxycycline inhibits α-synuclein-associated pathologies in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Dominguez-Meijide,Valeria Parrales,Eftychia Vasili,Florencia González-Lizárraga,Annekatrin König,Diana F Lázaro,Annie Lannuzel,Stéphane Haik,Elaine Del Bel,Rosana Chehín,Rita Raisman-Vozari,Patrick P Michel,Nicolas Bizat,Tiago Fleming Outeiro

Journal of neurochemistry 156:674-691 PubMed32730640

2020

Dopamine promotes the neurodegenerative potential of β-synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Anupam Raina,Kristian Leite,Sofia Guerin,Sameehan U Mahajani,Kalyan S Chakrabarti,Diana Voll,Stefan Becker,Christian Griesinger,Mathias Bähr,Sebastian Kügler

Cell death & disease 10:898 PubMed31776327

2019

Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Sameehan Mahajani,Anupam Raina,Claudia Fokken,Sebastian Kügler,Mathias Bähr

The Journal of biological chemistry 294:9973-9984 PubMed31092553

2019

C-terminal α-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan P McGlinchey,Shannon M Lacy,Katherine E Huffer,Nahid Tayebi,Ellen Sidransky,Jennifer C Lee

Neurobiology of disease 124:276-288 PubMed30381260

2018

Development of an aggregate-selective, human-derived α-synuclein antibody BIIB054 that ameliorates disease phenotypes in Parkinson's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Weihofen,YuTing Liu,Joseph W Arndt,Christian Huy,Chao Quan,Benjamin A Smith,Jean-Luc Baeriswyl,Nicole Cavegn,Luzia Senn,Lihe Su,Galina Marsh,Pavan K Auluck,Fabio Montrasio,Roger M Nitsch,Warren D Hirst,Jesse M Cedarbaum,R Blake Pepinsky,Jan Grimm,Paul H Weinreb

PloS one 12:e0184262 PubMed28877262

2017

The ER retention protein RER1 promotes alpha-synuclein degradation via the proteasome.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo-Jin Park,Daniel Ryu,Mayur Parmar,Benoit I Giasson,Nikolaus R McFarland

Human molecular genetics 25:275-90 PubMed26586132

2015

Yeast reveals similar molecular mechanisms underlying alpha- and beta-synuclein toxicity.

Applications

ICC/IF

Species

Human

Sandra Tenreiro,Rita Rosado-Ramos,Ellen Gerhardt,Filippo Favretto,Filipa Magalhães,Blagovesta Popova,Stefan Becker,Markus Zweckstetter,Gerhard H Braus,Tiago Fleming Outeiro

Journal of neuropathology and experimental neurology 72:624-45 PubMed23771222

2013

α-Synuclein vaccination prevents the accumulation of parkinson disease-like pathologic inclusions in striatum in association with regulatory T cell recruitment in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Vanesa Sanchez-Guajardo,Ambra Annibali,Poul Henning Jensen,Marina Romero-Ramos

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Anti-pan Synuclein antibody

Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (AB6176)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (18)

Product promise