AB314907

Anti-Pan Trk antibody [RM1032] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Multiclonal TrkC antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell line samples.

대체 명칭 보기

TRKC, NTRK3, NT-3 growth factor receptor, GP145-TrkC, Neurotrophic tyrosine kinase receptor type 3, TrkC tyrosine kinase, Trk-C, TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B, MTC, TRK, TRKA, NTRK1, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, Trk-A

17 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with TrkC expression vector containing a His-tag. (B) HEK-293T transfected with TrkA expression vector containing a His-tag. (C) HEK-293T transfected with TrkB expression vector containing a His-tag. (D) HEK-293T transfected with an empty vector containing a His-tag. Tissue labeling Pan Trk with ab314906 at 1/1000 (0.489 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with TrkC expression vector containing a His-tag, (B) HEK-293T transfected with TrkA expression vector containing a His-tag, and (C) HEK-293T transfected with TrkB expression vector containing a His-tag. No staining on (D) HEK-293T transfected with an empty vector containing a His-tag. The section was incubated with ab314906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized IMR-32 (human neuroblastoma neuroblast) cells labelling Pan Trk with ab314906 at 1/200 (2.445 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in IMR-32 cell line. Negative control : THP-1. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Pan Trk with ab314906 at 1/500 (0.978 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human kidney. The section was incubated with ab314906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Pan Trk with ab314906 at 1/500 (0.978 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab314906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunoprecipitation - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Pan Trk was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate with ab314906 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314906 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate Lane 2 : ab314906 IP in IMR-32 (human neuroblastoma neuroblast) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314906 in IMR-32 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Pan Trk antibody [RM1032] (<a href='/ko/products/primary-antibodies/pan-trk-antibody-rm1032-ab314906'>ab314906</a>) at 1/30 dilution

All lanes:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 84s

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Pan Trk with ab314906 at 1/200 (2.445 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neural/glia cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized rat primary neuron cell cells labelling Pan Trk with ab314906 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Pan Trk with ab314906 at 1/1000 (0.489 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse kidney. The section was incubated with ab314906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Pan Trk with ab314906 at 1/1000 (0.489 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab314906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Pan Trk with ab314906 at 1/1000 (0.489 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab314906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Pan Trk with ab314906 at 1/200 (2.445 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in rat primary neural/glia cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neuron cells cells labelling Pan Trk with ab314906 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Pan Trk was immunoprecipitated from 0.35 mg rat brain tissue lysate with ab314906 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314906 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Rat brain tissue lysate Lane 2 : ab314906 IP in Rat brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314906 in rat brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Pan Trk antibody [RM1032] (<a href='/ko/products/primary-antibodies/pan-trk-antibody-rm1032-ab314906'>ab314906</a>) at 1/30 dilution

All lanes:

Rat brain tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

Supplier Data

Immunoprecipitation - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Pan Trk was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab314906 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314906 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse brain tissue lysate Lane 2 : ab314906 IP in Mouse brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314906 in mouse brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Pan Trk antibody [RM1032] (<a href='/ko/products/primary-antibodies/pan-trk-antibody-rm1032-ab314906'>ab314906</a>) at 1/30 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

Supplier Data

Western blot - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

All lanes:

Western blot - Anti-Pan Trk antibody [RM1032] (<a href='/ko/products/primary-antibodies/pan-trk-antibody-rm1032-ab314906'>ab314906</a>) at 1/1000 dilution

Lane 1:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 140 kDa,32 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : heart, kidney, spleen (PMID : 7823156). TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the observed Mw of ~120KDa is higher than the predicted Mw due to glycosylation (PMID : 24860020). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Pan Trk antibody [RM1032] (<a href='/ko/products/primary-antibodies/pan-trk-antibody-rm1032-ab314906'>ab314906</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Lane 5:

Mouse brain tissue lysate at 20 µg

Lane 6:

Mouse heart tissue lysate at 20 µg

Lane 7:

Mouse kidney tissue lysate at 20 µg

Lane 8:

Mouse spleen tissue lysate at 20 µg

Lane 9:

Rat brain tissue lysate at 20 µg

Lane 10:

Rat heart tissue lysate at 20 µg

Lane 11:

Rat kidney tissue lysate at 20 µg

Lane 12:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 140 kDa,32 kDa,36 kDa

false

Exposure time: 70s

Supplier Data

Western blot - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

This data was developed using ab314906, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Samples are non-boiled as boiling may cause protein aggregation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Pan Trk antibody [RM1032] (<a href='/ko/products/primary-antibodies/pan-trk-antibody-rm1032-ab314906'>ab314906</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with human TrkA expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 3:

293T transfected with human TrkB expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with human TrkC expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 120 kDa,36 kDa

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1032

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IP, WB, Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab314907 is the carrier-free version of ab314906.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pan Trk also known as Pan-tropomyosin receptor kinases are receptors that belong to the neurotrophin receptor family including TrkA TrkB and TrkC. They typically express in neural tissues and play a role in neuronal development and function. The mass of Pan Trk can vary as it encompasses several related proteins rather than a single unit. The variant forms are present in both the central and peripheral nervous systems. Researchers often use tags like Pan Trk IHc 555 Pan and Alexa Pan to identify different forms and monitor their expression patterns.

Biological function summary

Pan Trk receptors play a vital role in the survival development and function of neurons by mediating the action of neurotrophins. They participate as part of a receptor complex activated by ligand binding which triggers autophosphorylation. These receptors modulate various processes like neuronal survival and differentiation. Pan Trk signals generally initiate upon binding neurotrophins leading to multiple intracellular events.

Pathways

Pan Trk receptors are central players in signaling pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways significantly impact cell survival and proliferation. Pan Trk often interacts with proteins like SHC and GRB2 to transmit signals downstream efficiently. These interactions are important for linking the surface receptor activation to the intracellular responses that govern cell growth and survival.

Dysregulation or mutations in Pan Trk pathways contribute to neurodegenerative diseases and cancers. For example in neuroblastoma altered Pan Trk expression can modify cell survival signals resulting in tumor growth. These receptors also relate to Alzheimer's disease where Pan Trk dysfunction can affect neuronal resilience and memory pathways. In these disorders Pan Trk associates with various proteins including Tau which can disrupt normal neuronal processes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation.

See full target information NTRK3

Additional targets

NTRK2,NTRK1

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Product promise

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Anti-Pan Trk antibody [RM1032] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunoprecipitation - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunocytochemistry/ Immunofluorescence - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunoprecipitation - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Immunoprecipitation - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Western blot - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Western blot - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

Western blot - Anti-Pan Trk antibody [RM1032] - BSA and Azide free (AB314907)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

Product promise