AB241505

Anti-Pan2 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal PAN2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PAN2 aa 1-50.

대체 명칭 보기

KIAA0710, USP52, PAN2, PAN2-PAN3 deadenylation complex catalytic subunit PAN2, Inactive ubiquitin carboxyl-terminal hydrolase 52, PAB1P-dependent poly(A)-specific ribonuclease, Poly(A)-nuclease deadenylation complex subunit 2, PAN deadenylation complex subunit 2

2 이미지

Supplier Data

Immunoprecipitation - Anti-Pan2 antibody (AB241505)

Pan2 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241505 used for IP at 6 μg per reaction. For WB 1 μg/ml.

Lane 1 : ab241505 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEWK-293T whole cell lysate.

Chemiluminescence detection : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Pan2 antibody (ab241505)

Predicted band size: 135 kDa

false

Supplier Data

Western blot - Anti-Pan2 antibody (AB241505)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Pan2 antibody (ab241505) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 135 kDa

false

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PAN2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q504Q3

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to Pan2 immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pan2 is a protein also known as PAN2 poly(A) specific ribonuclease or PAN2-1. It functions as an essential component of the Pan2-Pan3 deadenylation complex responsible for shortening poly(A) tails of mRNAs. This action plays an important role in mRNA turnover and regulation of gene expression. Pan2 has a mass of approximately 162 kDa. Scientists have observed its expression in various tissues with notable presence in the cytoplasm where it interacts with other elements of the mRNA decay machinery.

Biological function summary

The targeted actions of Pan2 facilitate the regulation of mRNA stability which influences protein synthesis rates. Pan2 is part of the Pan2-Pan3 complex where it collaborates with Pan3 to catalyze the initial steps of deadenylation. This process is critical for normal cellular function affecting mRNA lifespan and its translation into protein. In this complex Pan2 provides the catalytic activity while Pan3 enhances its function and anchors the complex to the mRNA substrate.

Pathways

The function of Pan2 in mRNA degradation places it in the mRNA surveillance pathway and the general mRNA decay pathway. These pathways ensure proper regulation and quality control of mRNA within the cell. Pan2 works closely with the CCR4-NOT complex a significant player in mRNA deadenylation. These interactions allow for efficient control over gene expression by modulating the stability and availability of mRNA for translation.

The dysregulation of Pan2 has been implicated in cancer and neurodegenerative diseases. Abnormal mRNA decay processes related to malfunctioning Pan2 can lead to uncontrolled cell growth and tumorigenesis. Additionally Pan2 malfunction may connect to proteins like NOT1 influential in other disorders for instance neurodegeneration. These associations highlight the protein's potential role in pathologies necessitating continued research for better understanding and treatment strategies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenylation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation.

See full target information PAN2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular cell 83:2290-2302.e13 PubMed37295431

2023

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Höpfler,Eva Absmeier,Sew-Yeu Peak-Chew,Evangelia Vartholomaiou,Lori A Passmore,Ivana Gasic,Ramanujan S Hegde

International journal of molecular sciences 23: PubMed36233342

2022

RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xu,Pengfei Shi,Fanwei Xia,Xuan Zhao,Junfan Chen,Rui Geng,Hongjuan Cui,Liqun Yang

Nature communications 11:5362 PubMed33097710

2020

USP52 regulates DNA end resection and chemosensitivity through removing inhibitory ubiquitination from CtIP.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Gao,Guijie Guo,Jinzhou Huang,Jake A Kloeber,Fei Zhao,Min Deng,Xinyi Tu,Wootae Kim,Qin Zhou,Chao Zhang,Ping Yin,Kuntian Luo,Zhenkun Lou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pan2 antibody

Immunoprecipitation - Anti-Pan2 antibody (AB241505)

Western blot - Anti-Pan2 antibody (AB241505)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise