AB213557

Anti-Parathyroid Hormone antibody [EPR20598]

RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal pth antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR20598] (AB213557)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR20598] (AB213557)

Immunohistochemical analysis of paraffin-embedded rat parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051 ready to use). Granular cytoplasmic staining on chief cells of rat parathyroid is observed no staining on adjacent thyroid cells (PMID : 7615802). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Parathyroid Hormone antibody [EPR20598] (AB213557)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Parathyroid Hormone antibody [EPR20598] (AB213557)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution. Granular cytoplasmic staining on chief cells of mouse parathyroid is observed no staining on adjacent thyroid cells (PMID : 7615802). The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) ab150077 at 1/1000 dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Parathyroid Hormone antibody [EPR20598] (AB213557)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized rat parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution. Granular cytoplasmic staining on chief cells of rat parathyroid is observed no staining on adjacent thyroid cells (PMID : 7615802). The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) ab150077 at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR20598] (AB213557)

Immunohistochemical analysis of paraffin-embedded mouse parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051 ready to use). Granular cytoplasmic staining on chief cells of mouse parathyroid is observed no staining on adjacent thyroid cells (PMID : 7615802). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20598

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab213557 has non-specific staining on mouse and rat kidney

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Hamster": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.

Biological function summary

PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.

Pathways

PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.

PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information pth

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Renal failure 47:2513677 PubMed40485166

2025

Dexmedetomidine improves septic acute kidney injury by inhibiting inflammation and oxidative stress through the activation of the Pink1/Park2 autophagy pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuxia Liao,Zhi Feng,Hairong Lin,Ye Zhou,Xinxin Lin,Xiao Lin,Huichang Zhuo

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305913 PubMed38059822

2023

Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Jiao Tian,Zhi-Jie Yin,Zhen-Jiang Li,Zhen-Zhen Wang,Zhen Xing,Chun-Yan Liu,Lin-Tao Wang,Chun-Ming Wang,Jun-Feng Zhang,Lei Dong

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Parathyroid Hormone antibody [EPR20598]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR20598] (AB213557)

Immunohistochemistry (Frozen sections) - Anti-Parathyroid Hormone antibody [EPR20598] (AB213557)

Immunohistochemistry (Frozen sections) - Anti-Parathyroid Hormone antibody [EPR20598] (AB213557)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR20598] (AB213557)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise