AB75150

Anti-Parathyroid Hormone Receptor 1/PTH1R antibody

Name: Anti-Parathyroid Hormone Receptor 1/PTH1R antibody
Brand: Abcam Limited
SKU: AB75150
Rating: 3 (3 reviews)

(3 리뷰들)

(10 제품이 사용된 논문 )

Rabbit Polyclonal Parathyroid Hormone Receptor 1/PTH1R antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 10 publications.

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Immunohistochemistry analysis of Parathyroid Hormone Receptor 1/PTH1R in paraffin-embedded human brain tissue using ab75150, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Immunofluorescence analysis of Parathyroid Hormone Receptor 1/PTH1R in MCF7 cells using ab75150, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

IHC-P

AbReview42550****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

ab75150 staining Parathyroid Hormone Receptor 1/PTH1R in Lizard regenerated tail (Anolis sagrei) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 45 minutes at 22°C; antigen retrieval was by heat mediation in sodium citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in 1% horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

Image is of a regenerated lizard tail cartilage tube, at a proximal region that has begun to ossify. Positive staining was observed in structures resembling embryonic bone collar and early hypertrophic chondrocytes.

This image is courtesy of an Abreview submitted by Thomas Lozito

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Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

All lanes:

Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells at 5 µg

Lane 2:

Extracts from Jurkat cells at 5 µg with immunising peptide

Predicted band size: 66 kDa

Observed band size: 117 kDa,56 kDa,82 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid Hormone Receptor 1 (PTH1R) also known as the parathyroid receptor or hormone receptor is a G-protein-coupled receptor with an approximate molecular mass of 66 kDa. This receptor is found in various tissues mainly in bone and kidney. PTH1R interacts with parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHrP) both of which play significant roles in maintaining calcium homeostasis. The receptor is an important regulator in these processes facilitating the signal transduction that governs important physiological responses.

Biological function summary

PTH1R facilitates the regulation of calcium and phosphate balance in the body. It does not act alone but participates as a part of a signaling complex engaging with intracellular G-proteins to initiate signaling cascades. The receptor's activity helps modulate bone remodeling by affecting osteoblast and osteoclast activity which are essential for bone development and mineralization. Moreover in the kidney PTH1R adjusts calcium reabsorption and phosphate excretion supporting the maintenance of serum calcium levels.

Pathways

PTH1R plays a significant role in the cyclic AMP (cAMP) and phosphatidylinositol signaling pathways. When PTH or PTHrP binds to PTH1R it activates the adenylate cyclase increasing cAMP levels and subsequently activating protein kinase A (PKA). This pathway affects various physiological processes like gene expression and metabolic regulation. In addition PTH1R interacts with the phospholipase C pathway facilitating calcium mobilization inside cells. Proteins like Gs and Gq are involved in these pathways aiding in the receptor's ability to exert its biological effects.

PTH1R is associated with conditions such as osteoporosis and hypoparathyroidism. Osteoporosis involves abnormal bone loss and malfunctioning of the receptor can exacerbate this condition by impairing normal bone remodeling. In hypoparathyroidism insufficient signaling through PTH1R leads to low levels of calcium affecting bone and other bodily functions. The receptor's interaction with proteins such as parathyroid hormone-related peptide further extends its relevance to these conditions as alterations in these proteins can influence disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

G-protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH) (PubMed : 10913300, PubMed : 18375760, PubMed : 19674967, PubMed : 27160269, PubMed : 30975883, PubMed : 35932760, PubMed : 8397094). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP) (PubMed : 30975883, PubMed : 35932760). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed : 20172855, PubMed : 30975883, PubMed : 35932760). PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH (PubMed : 35932760).

See full target information PTH1R

대체 명칭 보기

PTHR, PTHR1, PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor, PTH/PTHrP type I receptor, Parathyroid hormone 1 receptor, PTH/PTHr receptor, PTH1 receptor

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제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Medicine 101:e32009 PubMed36482540

2022

Production of parathyroid-like cells from thyroid stem cells in co-culture environment.

Applications

Unspecified application

Species

Unspecified reactive species

Özge Karabiyik Acar,Gülçin Delal Nozhatzadeh,Alperen Tuncer,Gamze Torun Köse,Ezgi Hacihasanoğlu,Fikrettin Sahin,Erhan Aysan

International journal of oral science 14:37 PubMed35853862

2022

PTHrP promotes subchondral bone formation in TMJ-OA.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhang,Caixia Pi,Chen Cui,Yang Zhou,Bo Liu,Juan Liu,Xin Xu,Xuedong Zhou,Liwei Zheng

Oxidative medicine and cellular longevity 2022:9955677 PubMed35265269

2022

Exogenous Parathyroid Hormone Alleviates Intervertebral Disc Degeneration through the Sonic Hedgehog Signalling Pathway Mediated by CREB.

Applications

Unspecified application

Species

Unspecified reactive species

You Li,Yifan Wei,He Li,Hui Che,Dengshun Miao,Cheng Ma,Yongxin Ren

Biochemistry and biophysics reports 27:101054 PubMed34189282

2021

CFTR-mediated anion secretion in parathyroid hormone-treated Caco-2 cells is associated with PKA and PI3K phosphorylation but not intracellular pH changes or Na/K-ATPase abundance.

Applications

Unspecified application

Species

Unspecified reactive species

Rattana Chaimana,Jarinthorn Teerapornpuntakit,Walailak Jantarajit,Kornkamon Lertsuwan,Saowalak Krungchanuchat,Nattapon Panupinthu,Nateetip Krishnamra,Narattaphol Charoenphandhu

Journal of cellular physiology 235:1480-1493 PubMed31301073

2019

Preactivation of β-catenin in osteoblasts improves the osteoanabolic effect of PTH in type 1 diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sixu Chen,Lei Yang,Sihao He,Jiazhi Yang,Daocheng Liu,Quanwei Bao,Hao Qin,Wenqiong Du,Xin Zhong,Can Chen,Zhaowen Zong

The American journal of pathology 188:1374-1388 PubMed29577935

2018

Parathyroid Hormone-Like Hormone Induces Epithelial-to-Mesenchymal Transition of Intestinal Epithelial Cells by Activating the Runt-Related Transcription Factor 2.

Applications

Unspecified application

Species

Unspecified reactive species

Shuying He,Minmin Xue,Cuiping Liu,Fang Xie,Lan Bai

PloS one 9:e89352 PubMed24551245

2014

Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling.

Applications

WB, ICC/IF

Species

Mouse, Mouse

Jianjun Hu,Jie Lu,Gewei Lian,Jingping Zhang,Jonathan L Hecht,Volney L Sheen

Osteoarthritis and cartilage 20:1197-207 PubMed22750747

2012

Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen.

Applications

Unspecified application

Species

Unspecified reactive species

M Horie,H Choi,R H Lee,R L Reger,J Ylostalo,T Muneta,I Sekiya,D J Prockop

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 30:877-84 PubMed22102368

2011

PTHrP increases RANKL expression by stromal cells from giant cell tumor of bone.

Applications

Unspecified application

Species

Unspecified reactive species

Robert W Cowan,Gurmit Singh,Michelle Ghert

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 26:2911-22 PubMed21915903

2011

Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen R Bond,Alice Lau,Silvia Penuela,Arthur V Sampaio,T Michael Underhill,Dale W Laird,Christian C Naus

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Parathyroid Hormone Receptor 1/PTH1R antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Immunocytochemistry/ Immunofluorescence - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (10)

Product promise