Anti-PARP12 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-PARP12 antibody (AB241967)

PARP12 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab241967 used for IP at 6 μg per reaction. For WB 1 μg/ml.

Lane 1 : ab241967 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-PARP12 antibody (ab241967)

Predicted band size: 79 kDa

false

• WB

Supplier Data

Western blot - Anti-PARP12 antibody (AB241967)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PARP12 antibody (ab241967) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 79 kDa

false

Exposure time: 1s