AB32895

Anti-Parvalbumin antibody

Name: Anti-Parvalbumin antibody
Brand: Abcam Limited
SKU: AB32895
Rating: 5 (8 reviews)

(8 리뷰들)

(14 제품이 사용된 논문 )

Goat Polyclonal Parvalbumin antibody. Suitable for WB, Flow Cyt and reacts with Human, Rat samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human PVALB aa 50 to C-terminus.

2 이미지

Flow Cytometry - Anti-Parvalbumin antibody (AB32895)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Parvalbumin antibody (AB32895)

Flow cytometric analysis of human peripheral blood monocytes. Primary incubation 30 minutes (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL).

Supplier Data

Western blot - Anti-Parvalbumin antibody (AB32895)

Lysate in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Parvalbumin antibody (ab32895) at 0.01 µg/mL

Lane 1:

Human cerebellum tissue lysate at 35 µg

Lane 2:

Human cerebellum tissue lysate + peptide at 35 µg

Lane 3:

Rat brain tissue lysate at 35 µg

Lane 4:

Rat brain tissue lysate + peptide at 35 µg

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주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human PVALB aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P20472

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.3 µg/mL", "WB-species-notes": "Block using milk. Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3μg/ml.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.3 µg/mL", "WB-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Parvalbumin sometimes referred to as PV is a calcium-binding protein approximately 12 kDa in size. It is recognized for its roles in muscle and neuronal tissues. Within the nervous system parvalbumin is prominently expressed in fast-spiking GABAergic interneurons while in muscle tissues it is present in fast-twitch muscle fibers. Other popular descriptors include anti PV and parvalbumins. These proteins play important roles in the regulation of cellular calcium levels.

Biological function summary

Parvalbumin helps modulate calcium ion concentration which is key in muscle relaxation and neurotransmission. It acts by buffering calcium ions and is not part of a larger protein complex. By impacting calcium transients parvalbumin affects processes like synaptic plasticity and the rapid contractions of muscle fibers. This is especially significant in the brain where rapid neuronal firing is needed and in muscles where quick recovery is essential.

Pathways

Parvalbumin is intimately linked with calcium signaling pathways as well as the regulation of muscle contraction. It ensures proper calcium ion homeostasis thereby aiding muscle relaxation after contraction. In the nervous system calbindin proteins parallel some of parvalbumin's functions participating in calcium ion buffering and modulation of neuron excitability. The balanced action of these proteins helps maintain cellular functions in both neurons and muscles.

Insufficient parvalbumin expression may link to epilepsy and schizophrenia. Deficits in this protein could interfere with normal neurotransmission and muscle relaxation cycles. Parvalbumin deficiency influences neurotransmitter release affecting synaptic transmission which may exacerbate symptoms in these conditions. In disease mechanisms proteins such as calbindin may indirectly relate with parvalbumin showing similar patterns of dysregulation and compensating for its absence in calcium handling.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.

See full target information PVALB

대체 명칭 보기

Parvalbumin alpha, Alpha-parvalbumin, Alpha-PV, PVALB

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제품이 사용된 논문 (14)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biological psychiatry global open science 4:100338 PubMed39099729

2024

Degradation of Perineuronal Nets in the Ventral Hippocampus of Adult Rats Recreates an Adolescent-Like Phenotype of Stress Susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

Débora A E Colodete,Anthony A Grace,Francisco S Guimarães,Felipe V Gomes

Basic & clinical pharmacology & toxicology 134:614-628 PubMed38426366

2024

Perineuronal nets as regulators of parvalbumin interneuron function: Factors implicated in their formation and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Thamyris Santos-Silva,Debora A E Colodete,João Roberto Fernandes Lisboa,Ícaro Silva Freitas,Caio Fábio Baeta Lopes,Victor Hadera,Thaís Santos Almeida Lima,Adriana Jesus Souza,Felipe V Gomes

Biomedical optics express 12:3684-3699 PubMed34221688

2021

Large-scale, cell-resolution volumetric mapping allows layer-specific investigation of human brain cytoarchitecture.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Costantini,Giacomo Mazzamuto,Matteo Roffilli,Annunziatina Laurino,Filippo Maria Castelli,Mattia Neri,Giovanni Lughi,Andrea Simonetto,Erica Lazzeri,Luca Pesce,Christophe Destrieux,Ludovico Silvestri,Valerio Conti,Renzo Guerrini,Francesco Saverio Pavone

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:12239-12254 PubMed33000527

2020

Tau knockout exacerbates degeneration of parvalbumin-positive neurons in substantia nigra pars reticulata in Parkinson's disease-related α-synuclein A53T mice.

Applications

Unspecified application

Species

Unspecified reactive species

Luyan Jiao,Meige Zheng,Jinhai Duan,Ting Wu,Zhao Li,Lin Liu,Xianhong Xiang,Xiaolu Tang,Jinyang He,Xingjian Li,Guofeng Zhang,Jinhui Ding,Huaibin Cai,Xian Lin

Journal of applied physiology (Bethesda, Md. : 198 128:1153-1162 PubMed32213111

2020

A chronic high-fat diet exacerbates contractile dysfunction with impaired intracellular Ca release capacity in the skeletal muscle of aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Eshima,Yoshifumi Tamura,Saori Kakehi,Ryo Kakigi,Ryota Hashimoto,Katsuhiko Funai,Ryuzo Kawamori,Hirotaka Watada

Journal of neurophysiology 123:1536-1551 PubMed32186432

2020

Contrast gain control occurs independently of both parvalbumin-positive interneuron activity and shunting inhibition in auditory cortex.

Applications

Unspecified application

Species

Unspecified reactive species

James E Cooke,Martin C Kahn,Edward O Mann,Andrew J King,Jan W H Schnupp,Ben D B Willmore

Proceedings of the National Academy of Sciences of 116:8010-8017 PubMed30936319

2019

Pejvakin-mediated pexophagy protects auditory hair cells against noise-induced damage.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Defourny,Alain Aghaie,Isabelle Perfettini,Paul Avan,Sedigheh Delmaghani,Christine Petit

Journal of applied physiology (Bethesda, Md. : 198 126:170-182 PubMed30433865

2018

Dysfunction of muscle contraction with impaired intracellular Ca handling in skeletal muscle and the effect of exercise training in male db/db mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Eshima,Yoshifumi Tamura,Saori Kakehi,Kyoko Nakamura,Nagomi Kurebayashi,Takashi Murayama,Ryo Kakigi,Takashi Sakurai,Ryuzo Kawamori,Hirotaka Watada

Neuropharmacology 143:79-94 PubMed30240784

2018

Orexin 2 receptor stimulation enhances resilience, while orexin 2 inhibition promotes susceptibility, to social stress, anxiety and depression.

Applications

Unspecified application

Species

Unspecified reactive species

Clarissa D Staton,Jazmine D W Yaeger,Delan Khalid,Fadi Haroun,Belissa S Fernandez,Jessica S Fernandez,Bali K Summers,Tangi R Summers,Monica Sathyanesan,Samuel S Newton,Cliff H Summers

American journal of physiology. Regulatory, integr 312:R1017-R1028 PubMed28438761

2017

Improved skeletal muscle Ca regulation in vivo following contractions in mice overexpressing PGC-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Eshima,Shinji Miura,Nanami Senoo,Koji Hatakeyama,David C Poole,Yutaka Kano

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Parvalbumin antibody

Flow Cytometry - Anti-Parvalbumin antibody (AB32895)

Western blot - Anti-Parvalbumin antibody (AB32895)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (14)

Product promise