Anti-Patched / PTCH1 antibody [EPR26072-78]

• WB

Supplier Data

Western blot - Anti-Patched / PTCH1 antibody [EPR26072-78] (AB325234)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative : HEL, 786-O.

PTCH1 is a glycosylated protein and can be deglycosylated by Protein Deglycosylation MIX II.

In Western blot, Anti-alpha Actinin 4 antibody [EPR2533 (2)] - Loading Control (ab108198) staining at 1/100,000 dilution.

All lanes:

Western blot - Anti-Patched / PTCH1 antibody [EPR26072-78] (ab325234) at 1/1000 dilution

Lane 1:

Untreated 22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

22Rv1 whole cell lysate treated with Protein Deglycosylation MIX II at 20 µg

Lane 3:

HEL (human erythroleukemia erythroblast) whole cell lysate at 20 µg

Lane 4:

786-O (human kidney epithelial cell) whole cell lysate at 20 µg

Lane 5:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 160-180 kDa,105 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Patched / PTCH1 antibody [EPR26072-78] (AB325234)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] - (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Patched / PTCH1 antibody [EPR26072-78] (ab325234) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 2:

293T transfected with PTCH1 (WT) expression vector containing an EGFP-Myc-tag whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 160-180 kDa,124 kDa,200 kDa

false

Exposure time: 6s