AB52588

Anti-PAX5 antibody [mAbcam52588]

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Mouse Monoclonal PAX5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

1 이미지

Western blot - Anti-PAX5 antibody [mAbcam52588] (AB52588)

WB

Unknown

Western blot - Anti-PAX5 antibody [mAbcam52588] (AB52588)

All lanes:

Western blot - Anti-PAX5 antibody [mAbcam52588] (ab52588) at 1 µg/mL

Lane 1:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (blocked with 1% milk) at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (blocked with 1% Milk) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 45 kDa,50 kDa

true

Exposure time: 20min

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam52588

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB236272

Anti-PAX5 antibody [rPAX5/2060] - BSA and Azide free

Recombinant

0
0

Primary Antibodies

AB224660

Anti-PAX5 antibody [PAX5/2060]

0
0

Primary Antibodies

AB238030

Anti-PAX5 antibody [PCRP-PAX5-1B1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [PCRP-PAX5-1B1] (AB238030)

0
0

Primary Antibodies

AB238031

Anti-PAX5 antibody [PAX5/2595]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [PAX5/2595] (AB238031)

0
0

Primary Antibodies

AB211293

Anti-PAX5 antibody [1H9]

BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [1H9] (AB211293)

0
0

Primary Antibodies

AB202956

Biotin Anti-PAX5 antibody [EPR3730(2)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-PAX5 antibody [EPR3730(2)] (AB202956)

0
0

Primary Antibodies

AB15164

Anti-PAX5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody (AB15164)

5
2

Primary Antibodies

AB109443

Anti-PAX5 antibody [EPR3730(2)]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (AB109443)

5
4

Primary Antibodies

AB193556

Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (AB193556)

0
0

Primary Antibodies

AB237773

Anti-PAX5 antibody [SP34] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PAX5 antibody [SP34] - BSA and Azide free (AB237773)

0
0

Primary Antibodies

AB234402

Anti-PAX5 antibody [rPAX5/2060]

Recombinant

0
0

Primary Antibodies

AB183575

Anti-PAX5 antibody

Flow Cytometry - Anti-PAX5 antibody (AB183575)

0
0

Primary Antibodies

AB227635

Anti-PAX5 antibody [SP34] - ChIP Grade

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PAX5 antibody [SP34] - ChIP Grade (AB227635)

0
0

Primary Antibodies

AB202837

HRP Anti-PAX5 antibody [EPR3730(2)]

RabMAb|Recombinant

Western blot - HRP Anti-PAX5 antibody [EPR3730(2)] (AB202837)

0
0

Primary Antibodies

AB246754

PE Anti-PAX5 antibody [EPR3730(2)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-PAX5 antibody [EPR3730(2)] (AB246754)

0
0

보완 제품

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

5
133

Primary Antibodies

AB237456

Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y] (AB237456)

0
0

Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

5
23

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

4
80

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "3 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PAX5 also called B-cell-specific activator protein (BSAP) plays an important role in regulating gene expression during B-cell development. PAX5 is a transcription factor with an approximate mass of 50-55 kDa. It expresses mainly in B cells and in certain regions of the brain and testis. This protein binds DNA and controls the transcription of genes necessary for B-cell commitment and identity. Through processes like alternative splicing PAX5 generates multiple isoforms that contribute to various functional roles in its expressed locations.

Biological function summary

PAX5 regulates the differentiation and maintenance of B-lymphocytes. It controls various genes critical for B-cell lineage commitment while repressing those involved in other lineages like T-cells and myeloid cells. PAX5 is part of a larger transcriptional complex interacting with other transcription factors to perform its function. During B-cell maturation PAX5 activates a specific gene set that helps shape the B-cell receptor repertoire a process important for effective immune responses.

Pathways

PAX5 integrates into key pathways such as the B-cell receptor signaling and JAK-STAT pathways. These pathways regulate processes like cell proliferation differentiation and apoptosis. In the B-cell receptor signaling PAX5 works alongside proteins like CD19 and BTK facilitating the transmission of activation signals necessary for B-cell survival. Connections in the JAK-STAT pathway involve interactions that promote gene expression changes necessary for B-cell functional activity further modulating the immune system's readiness.

PAX5 is significantly associated with B-cell acute lymphoblastic leukemia (B-ALL) and non-Hodgkin lymphoma. In B-ALL mutations or deletions in PAX5 often lead to impaired B-cell development and uncontrolled cell proliferation. PAX5 mutations influence proteins like EBF1 and IL7R which are pivotal in B-cell progenitor activity and leukemogenesis. abnormalities in PAX5 expression or function can disrupt normal immune function leading to B-cell associated cancers and impairing immune surveillance highlighting its importance in maintaining immune system health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed : 10811620, PubMed : 27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed : 10811620, PubMed : 27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed : 32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed : 32612238).. (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed : 31941781). Also participates in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed : 23678172).

See full target information PAX5

대체 명칭 보기

Paired box protein Pax-5, B-cell-specific transcription factor, BSAP, PAX5

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 23:1056 PubMed41053808

2025

A novel anti-PD-L1/IL-8 bispecific antibody BP2402 enhances antitumor immunity and modulates inflammatory signaling in triple-negative breast cancer mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Song,Sumin Tang,Xuelei Pi,Yuanyuan Yan,Chenxi Hu,Liang Liu,Hongna Chen,Yating Zhang,Shishi Liu,Dan Yu,Chengkai Yin,Tianyan Liu,Xu Li,Deshan Li,Zhenzhong Wang,Wei Zhu,Kaiyuan Hui,Zhihang Liu,Xiaodong Jiang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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