Anti-PAX7 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (AB187339)

Immunofluorescent analysis of PAX7 in MCF7 (left panel) and HeLa (right panel) cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were probed with a ab187339 at 1/100 dilution for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (AB187339)

Immunocytochemistry analysis of formalin-fixed MCF-7 cells (human breast adenocarcinoma cell line) permeabilized with 0.1% Triton X-100 staining PAX-7 in the nucleus with ab187339 in a 1/100 dilution (green). Secondary was DyLight-conjugated secondary antibody. Negative control (left), had no primary antibody. F-actin was stianed with fluorescent red phalloidin, and nuclei were stained with DAPI or Hoechst.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (AB187339)

Immunocytochemistry analysis of formalin-fixed HeLa cells permeabilized with 0.1% Triton X-100 staining PAX-7 in the nucleus with ab187339 in a 1/100 dilution (green). Secondary was DyLight-conjugated secondary antibody. Negative control (left), had no primary antibody. F-actin was stianed with fluorescent red phalloidin, and nuclei were stained with DAPI or Hoechst.

• IP

Supplier Data

Immunoprecipitation - Anti-PAX7 antibody (AB187339)

Immunoprecipitation of PAX7 was performed using HeLa whole cell lysate. Antigen-antibody complexes were formed by incubating 500μg of lysate with 3μg of ab187339 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted. Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with ab187339 at 1/1000 dilution overnight rotating at 4°C.

All lanes:

Immunoprecipitation - Anti-PAX7 antibody (ab187339)

Predicted band size: 56 kDa

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• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (AB187339)

Immunocytochemistry analysis of formalin-fixed C2C12 cells (mouse myoblast cell line) permeabilized with 0.1% Triton X-100 staining PAX-7 in the nucleus with ab187339 in a 1/100 dilution (green). Secondary was DyLight-conjugated secondary antibody. Negative control (left), had no primary antibody. F-actin was stianed with fluorescent red phalloidin, and nuclei were stained with DAPI or Hoechst.

• WB

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Western blot - Anti-PAX7 antibody (AB187339)

All lanes:

Western blot - Anti-PAX7 antibody (ab187339) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

MCF7 whole cell lysate at 50 µg

Lane 3:

HSkMSC whole cell lysate at 50 µg

Lane 4:

SJCRH30 whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/20000 dilution

Predicted band size: 56 kDa

true

• WB

Supplier Data

Western blot - Anti-PAX7 antibody (AB187339)

All lanes:

Western blot - Anti-PAX7 antibody (ab187339) at 1/1000 dilution

Lane 1:

HeLa lysate transfected with empty vector control at 50 µg

Lane 2:

HeLa lysate overexpressing PAX7 at 50 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/20000 dilution

Predicted band size: 56 kDa

true