AB31833

Anti-Paxillin (phospho S83) antibody

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Rabbit Polyclonal Paxillin phospho S83 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Pxn phospho S83 conjugated to Keyhole Limpet Haemocyanin.

1 이미지

Western blot - Anti-Paxillin (phospho S83) antibody (AB31833)

WB

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Western blot - Anti-Paxillin (phospho S83) antibody (AB31833)

Western blot analysis of immunoprecipitated paxillin (arrow) in mouse macrophages untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min (lanes 2, 4, 5, & 6). Lysates were immunoprecipitated with anti-Paxillin. The blots were probed with anti-Paxillin or anti-phospho-Paxillin (Ser-83). The latter was used in the presence of phospho-Paxillin (Ser-83) peptide (lane 5) or phospho-Paxillin (Ser-85) peptide (lane 6). Immunoglobulin (Ig) chains from the immunoprecipitation were observed above and below the paxillin bands.

All lanes:

Western blot - Anti-Paxillin (phospho S83) antibody (ab31833)

Predicted band size: 65 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

Immunogen

Synthetic Peptide within Mouse Pxn phospho S83 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab31833 detects a 68kDa protein corresponding to the molecular mass of phosphorylated paxillin.

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대체품

Primary Antibodies

AB2264

Anti-Paxillin antibody

Western blot - Anti-Paxillin antibody (AB2264)

4
5

Primary Antibodies

AB109547

Anti-Paxillin (phospho Y118) antibody [EPR1903]

RabMAb|Recombinant

Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)

0
0

Primary Antibodies

AB32115

Anti-Paxillin antibody [E228]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [E228] (AB32115)

0
0

Primary Antibodies

AB216652

Anti-Paxillin antibody [Y113] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] - BSA and Azide free (AB216652)

0
0

Primary Antibodies

AB238950

Anti-Paxillin antibody [E228] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [E228] - BSA and Azide free (AB238950)

0
0

Primary Antibodies

AB32084

Anti-Paxillin antibody [Y113]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] (AB32084)

5
21

보완 제품

Primary Antibodies

AB32115

Anti-Paxillin antibody [E228]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [E228] (AB32115)

0
0

Primary Antibodies

AB229312

Anti-Nebulette antibody - C-terminal

Western blot - Anti-Nebulette antibody - C-terminal (AB229312)

0
0

Primary Antibodies

AB178876

Anti-MEK1 + MEK2 antibody [EPR16667]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 + MEK2 antibody [EPR16667] (AB178876)

5
1

Primary Antibodies

AB238137

Anti-IRF7 antibody [EPR23509-21]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-IRF7 antibody [EPR23509-21] (AB238137)

0
0

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>In 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Paxillin also known as PXN is a cytoskeletal protein involved in the assembly of focal adhesions. It has a molecular weight of approximately 68 kDa. You will find Paxillin expressed widely in various tissues including the liver colon and lung. Paxillin plays a part in cell motility by interacting with other focal adhesion proteins. It plays a significant mechanical role by serving as a docking site for structural and signaling molecules.

Biological function summary

Paxillin serves key functions in cellular processes such as migration proliferation and survival. It interacts with different proteins including vinculin and talin forming part of a complex at the focal adhesion sites. The phosphorylation state of Paxillin can modulate its interactions and in this way influence the assembly of signaling complexes that control dynamic cellular processes.

Pathways

Paxillin participates in the integrin signaling and MAPK pathways. It operates by linking integrin receptors to the actin cytoskeleton facilitating signal transduction. Paxillin phosphorylation is an important regulatory mechanism within these pathways. In particular its interaction with focal adhesion kinase (FAK) and Src family kinases signifies its role in transmitting signals from the extracellular matrix to the cellular interior which impacts cell behavior and response.

Paxillin has links with osteosarcoma and lung cancer. Deregulation of Paxillin expression or its phosphorylation state contributes to oncogenic transformation and tumor progression. In cancer Paxillin associates with altered activity of proteins such as Src and FAK exacerbating disease states. This relationship highlights Paxillin's potential as a biomarker and therapeutic target in treating related malignancies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.

See full target information Pxn phospho S83

대체 명칭 보기

Paxillin

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Molecular medicine reports 20:3131-3139 PubMed31432112

2019

Halofuginone protects against advanced glycation end products‑induced injury of H9C2 cells via alleviating endoplasmic reticulum stress‑associated apoptosis and inducing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hui Li,Wei-Li Zhang,Hao-Ying Zhou,Da-Wei Yu,Xiao-Ning Sun,Qin Hu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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