AB24786

Anti-Paxillin (phospho Y31) antibody [M102]

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Mouse Monoclonal Paxillin phospho Y31 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PXN pY31 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

Paxillin, PXN

1 이미지

Western blot - Anti-Paxillin (phospho Y31) antibody [M102] (AB24786)

WB

Unknown

Western blot - Anti-Paxillin (phospho Y31) antibody [M102] (AB24786)

All lanes:

Western blot - Anti-Paxillin (phospho Y31) antibody [M102] (ab24786)

Lane 1:

A431 cells (30 mg/lane) serum starved overnight

Lane 2:

A431 cells (30 mg/lane) treated with pervanadate (1 mMl) for 30 min.

Predicted band size: 65 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

M102

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

Immunogen

Synthetic Peptide within Human PXN pY31 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB2264

Anti-Paxillin antibody

Western blot - Anti-Paxillin antibody (AB2264)

4
5

Primary Antibodies

AB109547

Anti-Paxillin (phospho Y118) antibody [EPR1903]

RabMAb|Recombinant

Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)

0
0

Primary Antibodies

AB32115

Anti-Paxillin antibody [E228]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [E228] (AB32115)

0
0

Primary Antibodies

AB216652

Anti-Paxillin antibody [Y113] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] - BSA and Azide free (AB216652)

0
0

Primary Antibodies

AB238950

Anti-Paxillin antibody [E228] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [E228] - BSA and Azide free (AB238950)

0
0

Primary Antibodies

AB32084

Anti-Paxillin antibody [Y113]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] (AB32084)

5
21

보완 제품

Primary Antibodies

AB129002

Anti-Vinculin antibody [EPR8185] - Loading Control

20ul selling size|RabMAb|Recombinant|KO Validated

5
21

Primary Antibodies

AB32084

Anti-Paxillin antibody [Y113]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] (AB32084)

5
21

Primary Antibodies

AB81298

Anti-FAK (phospho Y397) antibody [EP2160Y]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

4
2

Primary Antibodies

AB109547

Anti-Paxillin (phospho Y118) antibody [EPR1903]

RabMAb|Recombinant

Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)

0
0

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Paxillin also known as PXN is a cytoskeletal protein involved in the assembly of focal adhesions. It has a molecular weight of approximately 68 kDa. You will find Paxillin expressed widely in various tissues including the liver colon and lung. Paxillin plays a part in cell motility by interacting with other focal adhesion proteins. It plays a significant mechanical role by serving as a docking site for structural and signaling molecules.

Biological function summary

Paxillin serves key functions in cellular processes such as migration proliferation and survival. It interacts with different proteins including vinculin and talin forming part of a complex at the focal adhesion sites. The phosphorylation state of Paxillin can modulate its interactions and in this way influence the assembly of signaling complexes that control dynamic cellular processes.

Pathways

Paxillin participates in the integrin signaling and MAPK pathways. It operates by linking integrin receptors to the actin cytoskeleton facilitating signal transduction. Paxillin phosphorylation is an important regulatory mechanism within these pathways. In particular its interaction with focal adhesion kinase (FAK) and Src family kinases signifies its role in transmitting signals from the extracellular matrix to the cellular interior which impacts cell behavior and response.

Paxillin has links with osteosarcoma and lung cancer. Deregulation of Paxillin expression or its phosphorylation state contributes to oncogenic transformation and tumor progression. In cancer Paxillin associates with altered activity of proteins such as Src and FAK exacerbating disease states. This relationship highlights Paxillin's potential as a biomarker and therapeutic target in treating related malignancies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.

See full target information PXN pY31

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology reports 45: PubMed33760159

2021

MicroRNA‑129 inhibits colorectal cancer cell proliferation, invasion and epithelial‑to‑mesenchymal transition by targeting SOX4.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiping Chen,Tianyu Zhong,Jinghua Zhong,Yang Tang,Baodian Ling,Lanfeng Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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