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AB131446

Anti-Paxillin (phospho Y88) antibody

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Rabbit Polyclonal Paxillin phospho Y88 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PXN pY88 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

Paxillin, PXN

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y88) antibody (AB131446)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y88) antibody (AB131446)

Immunofluorescent analysis of methanol-fixed HeLa cells labelling Paxillin (phospho Y88) with ab131446 at 1/100 dilution.

Western blot - Anti-Paxillin (phospho Y88) antibody (AB131446)
  • WB

Unknown

Western blot - Anti-Paxillin (phospho Y88) antibody (AB131446)

All lanes:

Western blot - Anti-Paxillin (phospho Y88) antibody (ab131446) at 1/500 dilution

Lane 1:

HT29 cell extract, untreated

Lane 2:

HT29 cell extract, treated with EGF

Predicted band size: 65 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human PXN pY88 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P49023

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대체품

Primary Antibodies

AB2264

Anti-Paxillin antibody

Western blot - Anti-Paxillin antibody (AB2264)

  • 4
  • 5
Primary Antibodies

AB109547

Anti-Paxillin (phospho Y118) antibody [EPR1903]

RabMAb|Recombinant

Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)

  • 0
  • 0
Primary Antibodies

AB32115

Anti-Paxillin antibody [E228]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [E228] (AB32115)

  • 0
  • 0
Primary Antibodies

AB216652

Anti-Paxillin antibody [Y113] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] - BSA and Azide free (AB216652)

  • 0
  • 0
Primary Antibodies

AB238950

Anti-Paxillin antibody [E228] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [E228] - BSA and Azide free (AB238950)

  • 0
  • 0
Primary Antibodies

AB32084

Anti-Paxillin antibody [Y113]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] (AB32084)

  • 5
  • 21

보완 제품

Primary Antibodies

AB227975

Anti-Lck antibody [EPR20798-107]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] (AB227975)

  • 0
  • 0
Primary Antibodies

AB313463

Anti-CCR2 antibody [RP23040162]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CCR2 Antibody [RP23040162] (AB313463)

  • 0
  • 0
Primary Antibodies

AB140779

Anti-CD64 antibody [OTI3D3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (AB140779)

  • 5
  • 3
Primary Antibodies

AB251662

Anti-Fibulin 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 2 antibody (AB251662)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab131446 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Paxillin also known as PXN is a cytoskeletal protein involved in the assembly of focal adhesions. It has a molecular weight of approximately 68 kDa. You will find Paxillin expressed widely in various tissues including the liver colon and lung. Paxillin plays a part in cell motility by interacting with other focal adhesion proteins. It plays a significant mechanical role by serving as a docking site for structural and signaling molecules.
Biological function summary

Paxillin serves key functions in cellular processes such as migration proliferation and survival. It interacts with different proteins including vinculin and talin forming part of a complex at the focal adhesion sites. The phosphorylation state of Paxillin can modulate its interactions and in this way influence the assembly of signaling complexes that control dynamic cellular processes.

Pathways

Paxillin participates in the integrin signaling and MAPK pathways. It operates by linking integrin receptors to the actin cytoskeleton facilitating signal transduction. Paxillin phosphorylation is an important regulatory mechanism within these pathways. In particular its interaction with focal adhesion kinase (FAK) and Src family kinases signifies its role in transmitting signals from the extracellular matrix to the cellular interior which impacts cell behavior and response.

Paxillin has links with osteosarcoma and lung cancer. Deregulation of Paxillin expression or its phosphorylation state contributes to oncogenic transformation and tumor progression. In cancer Paxillin associates with altered activity of proteins such as Src and FAK exacerbating disease states. This relationship highlights Paxillin's potential as a biomarker and therapeutic target in treating related malignancies.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.
See full target information PXN pY88
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Thoracic cancer 14:1543-1555 PubMed37073425

2023

Downregulation of circ_PLXND1 inhibits tumorigenesis of non-small cell lung carcinoma via miR-1287-5p/ERBB3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinzhou Wu,Chenyang Liu,Guiping Yu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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