AB242318

Anti-PBRM1/BAF180 antibody [CL0331]

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Mouse Monoclonal PBRM1/BAF180 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PBRM1 aa 100-250.

대체 명칭 보기

BAF180, PB1, PBRM1, Protein polybromo-1, hPB1, BRG1-associated factor 180, Polybromo-1D

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Paraffin-embedded human breast cancer tissue stained for PBRM1/BAF180 using ab242318 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Negative Control :

Paraffin-embedded human skeletal muscle tissue stained for PBRM1/BAF180 using ab242318 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Paraffin-embedded human endometrium tissue stained for PBRM1/BAF180 using ab242318 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Paraffin-embedded human kidney cancer tissue stained for PBRM1/BAF180 using ab242318 at 1/50 dilution in immunohistochemical analysis.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0331

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PBRM1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q86U86

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PBRM1 also known as BAF180 is an important component of the SWI/SNF chromatin remodeling complex. It has a molecular weight of approximately 187 kDa. Within the cell PBRM1 is expressed in various tissues but shows higher expression in kidney and breast tissues. Its role involves binding to acetylated histones which influences chromatin structure and gene expression regulation.

Biological function summary

The PBRM1/BAF180 protein functions as part of the SWI/SNF complex which includes the catalytic subunit BRG1 or BRM. This complex is important for altering chromatin architecture which regulates access of transcriptional machinery to DNA. It plays a significant role in the control of cell growth and differentiation processes. The structural integrity of PBRM1 is necessary for the functionality of the SWI/SNF complex.

Pathways

PBRM1/BAF180 is an important player in pathways involving chromatin remodeling and transcriptional regulation. It is implicated in the WNT signaling pathway interacting with proteins such as β-catenin to influence gene transcription. It also partakes in the p53 pathway where it contributes to the regulation of cell cycle and apoptosis by modulating p53 activity.

Mutations in PBRM1 are frequently observed in renal cell carcinoma. Its disruption affects chromatin remodeling which disrupts normal gene expression contributing to cancer progression. PBRM1 also relates to breast cancer where alterations can promote tumor formation. In these contexts the interaction of PBRM1 with other proteins like VHL in renal carcinoma helps elucidate its role in tumor suppression and development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation.

See full target information PBRM1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell proliferation 55:e13190 PubMed35102634

2022

Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Thao Thi Thanh Ngo,Bella Rossbach,Isabelle Sébastien,Julia C Neubauer,Andreas Kurtz,Krithika Hariharan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PBRM1/BAF180 antibody [CL0331]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [CL0331] (AB242318)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise