AB249470

Anti-PCBP1 antibody [EPR11055] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PCBP1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3, hnRNP E1, PCBP1

2 이미지

Lab

Immunoprecipitation - Anti-PCBP1 antibody [EPR11055] - BSA and Azide free (AB249470)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168378).
Purified ab168378 at 1 : 50 dilution (2µg) immunoprecipitating PCBP1 in 293T whole cell lysate.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PCBP1 antibody [EPR11055] (<a href='/ko/products/primary-antibodies/pcbp1-antibody-epr11055-ab168378'>ab168378</a>) at 1/50 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/pcbp1-antibody-epr11055-ab168378'>ab168378</a> + 293T whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/pcbp1-antibody-epr11055-ab168378'>ab168378</a> in 293T whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 37 kDa

false

Unknown

Western blot - Anti-PCBP1 antibody [EPR11055] - BSA and Azide free (AB249470)

All lanes:

Western blot - Anti-PCBP1 antibody [EPR11055] (<a href='/ko/products/primary-antibodies/pcbp1-antibody-epr11055-ab168378'>ab168378</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 37 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11055

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

ab249470 is the carrier-free version of ab168378.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The PCBP1 protein also known as Poly(rC) Binding Protein 1 or hnRNP E1 is a RNA-binding protein with a mass of approximately 39-40 kDa. Mechanically it binds to single-stranded poly(C) regions in RNA through its three K-homology (KH) domains. These domains allow PCBP1 to regulate mRNA stability translation and splicing. The protein is broadly expressed across many tissues with high levels in the liver thymus kidneys and the brain suggesting its essential involvement in diverse cellular processes.

Biological function summary

PCBP1 influences the cellular machinery through its role in post-transcriptional regulation. It does not function alone but rather exists as part of an RNA-protein complex. This protein participates in modulating mRNA turnover and translational silencing playing important roles in cellular iron homeostasis and response to viral infections. Through its interaction with specific mRNAs PCBP1 helps regulate processes like cell proliferation and differentiation where its dysregulation may have significant consequences.

Pathways

Alterations in PCBP1 activities impact important cellular pathways such as the Wnt signaling pathway and the iron metabolism pathway. In the Wnt signaling pathway PCBP1 interacts with other proteins like beta-catenin to regulate gene expression that guides cell growth and division. In iron metabolism PCBP1 plays a role by stabilizing iron-responsive element (IRE)-containing mRNAs therefore influencing the expression of proteins involved in iron storage and transport.

PCBP1 has connections to cancer and anemia. In various cancers such as colorectal and lung cancer PCBP1 expression levels change suggesting a role in tumor progression and metastasis. It interacts with proteins such as beta-catenin which is known to contribute to oncogenic pathways. In conditions of anemia particularly sideroblastic anemia PCBP1 influences the expression of proteins that are critical for iron regulation like ferritin affecting iron homeostasis in red blood cells. Understanding these relationships provides potential avenues for therapeutic interventions targeting PCBP1 and associated pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed : 15731341, PubMed : 7556077, PubMed : 7607214, PubMed : 8152927). Together with PCBP2, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity).. (Microbial infection) In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD.

See full target information PCBP1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCBP1 antibody [EPR11055] - BSA and Azide free

Immunoprecipitation - Anti-PCBP1 antibody [EPR11055] - BSA and Azide free (AB249470)

Western blot - Anti-PCBP1 antibody [EPR11055] - BSA and Azide free (AB249470)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise