AB72443

Anti-PCM1 antibody

Name: Anti-PCM1 antibody
Brand: Abcam Limited
SKU: AB72443
Rating: 4 (2 reviews)

(2 리뷰들)

(9 제품이 사용된 논문 )

Rabbit Polyclonal PCM1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human PCM1.

1 이미지

Unknown

Western blot - Anti-PCM1 antibody (AB72443)

Detection : Chemiluminescence with exposure times of 30 seconds

All lanes:

Western blot - Anti-PCM1 antibody (ab72443) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T cell lysate at 50 µg

Lane 5:

Mouse NIH3T3 cell lysate at 50 µg

Predicted band size: 229 kDa

Observed band size: 270 kDa,60 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PCM1. The exact immunogen used to generate this antibody is proprietary information.

Q15154

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PCM1 (Pericentriolar Material 1) also known as the PCM-1 protein functions mechanically in the organization of centrosomes and microtubules. This mechanistic role is linked with its name due to its positioning around the centrosome a critical cellular structure for division. The PCM1 protein shows an approximate mass of 228 kDa. It is expressed broadly in various tissues but mainly in organs with high cellular turnover including the testis thymus and heart. Notably PCM1 serves as a scaffold protein anchoring various centrosomal proteins important for their correct assembly and positioning.

Biological function summary

The PCM1 protein plays an important role in the microtubule stabilization and cell cycle progression. This protein becomes part of the larger pericentriolar material complex which supports the necessary structural platform for centrosome and cilia function. PCM1 assists in docking other proteins like centrin centrin-binding proteins and pericentriolar satellites in the complex ensuring the intricacies of ciliary trafficking and centrosome cohesion are well-maintained. It helps in regulating processes critical to normal cell polarity and division.

Pathways

PCM1 protein integrates pathway activities relating to not only microtubule dynamism but also cellular motility. It is involved in the centrosome-cilia interface important for signal transduction and cellular homeostasis. PCM1 interacts with key pathway proteins such as CEP290 and OFD1 which are significant for ciliogenesis thereby influencing cellular responses to extracellular cues. These pathways directly tie the mechanics of PCM1 to essential biological responses including cell signaling pathways that ensure normal embryonic development and cell differentiation.

PCM1 plays a relevant role in the context of ciliopathies and certain cancers. Abnormalities or mutations in PCM1 have associations with disorders such as Bardet-Biedl Syndrome a ciliopathy characterized by malfunctioning cilia. Aberrant PCM1 expressions or mutations also have links to leukemia through its implication in the ETV6-PCM1 fusion gene. The interplay between PCM1 and proteins such as ETV6 in leukemia highlights its impact on chromosomal translocations and the potential muscular pathway misregulation leading to malignancy.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for centrosome assembly and function (PubMed : 12403812, PubMed : 15659651, PubMed : 16943179). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed : 12403812, PubMed : 15659651). Required to anchor microtubules to the centrosome (PubMed : 12403812, PubMed : 15659651). Also involved in cilium biogenesis by recruiting the BBSome, a ciliary protein complex involved in cilium biogenesis, to the centriolar satellites (PubMed : 20551181, PubMed : 24121310, PubMed : 27979967). Recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites (PubMed : 34782749).

See full target information PCM1

대체 명칭 보기

Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 44:115091 PubMed39709602

2024

Transcriptional profile of the rat cardiovascular system at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Arduini,Stephen J Fleming,Ling Xiao,Amelia W Hall,Amer-Denis Akkad,Mark D Chaffin,Kayla J Bendinelli,Nathan R Tucker,Irinna Papangeli,Helene Mantineo,Patricio Flores-Bringas,Mehrtash Babadi,Christian M Stegmann,Guillermo García-Cardeña,Mark E Lindsay,Carla Klattenhoff,Patrick T Ellinor

iScience 27:109580 PubMed38600973

2024

Necrosulfonamide causes oxidation of PCM1 and impairs ciliogenesis and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Clotilde C N Renaud,Carolina Alves Nicolau,Clément Maghe,Kilian Trillet,Jane Jardine,Sophie Escot,Nicolas David,Julie Gavard,Nicolas Bidère

Communications biology 6:1271 PubMed38102401

2023

The centrosomal protein 131 participates in the regulation of mitochondrial apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Clotilde C N Renaud,Kilian Trillet,Jane Jardine,Laura Merlet,Ophélie Renoult,Mélanie Laurent-Blond,Zoé Catinaud,Claire Pecqueur,Julie Gavard,Nicolas Bidère

Cell reports 28:1907-1922.e6 PubMed31412255

2019

A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kari-Anne M Frikstad,Elisa Molinari,Marianne Thoresen,Simon A Ramsbottom,Frances Hughes,Stef J F Letteboer,Sania Gilani,Kay O Schink,Trond Stokke,Stefan Geimer,Lotte B Pedersen,Rachel H Giles,Anna Akhmanova,Ronald Roepman,John A Sayer,Sebastian Patzke

Molecular biology of the cell 29:1542-1554 PubMed29742019

2018

The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Robert F Shearer,Kari-Anne Myrum Frikstad,Jessie McKenna,Rachael A McCloy,Niantao Deng,Andrew Burgess,Trond Stokke,Sebastian Patzke,Darren N Saunders

Nature communications 8:14177 PubMed28134340

2017

KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth B Schou,Johanne B Mogensen,Stine K Morthorst,Brian S Nielsen,Aiste Aleliunaite,Andrea Serra-Marques,Nicoline Fürstenberg,Sophie Saunier,Albane A Bizet,Iben R Veland,Anna Akhmanova,Søren T Christensen,Lotte B Pedersen

eLife 4: PubMed26386247

2015

TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23).

Applications

ICC

Species

Unspecified reactive species

Louise A Stephen,Hasan Tawamie,Gemma M Davis,Lars Tebbe,Peter Nürnberg,Gudrun Nürnberg,Holger Thiele,Michaela Thoenes,Eugen Boltshauser,Steffen Uebe,Oliver Rompel,André Reis,Arif B Ekici,Lynn McTeir,Amy M Fraser,Emma A Hall,Pleasantine Mill,Nicolas Daudet,Courtney Cross,Uwe Wolfrum,Rami Abou Jamra,Megan G Davey,Hanno J Bolz

Cell cycle (Georgetown, Tex.) 14:1116-25 PubMed25830415

2015

Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ankur A Gholkar,Silvia Senese,Yu-Chen Lo,Joseph Capri,William J Deardorff,Harish Dharmarajan,Ely Contreras,Emmanuelle Hodara,Julian P Whitelegge,Peter K Jackson,Jorge Z Torres

Nature communications 5:5308 PubMed25374274

2014

Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents.

Applications

Species

Unspecified reactive species

Jantje M Gerdes,Sonia Christou-Savina,Yan Xiong,Tilo Moede,Noah Moruzzi,Patrick Karlsson-Edlund,Barbara Leibiger,Ingo B Leibiger,Claes-Göran Östenson,Philip L Beales,Per-Olof Berggren

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Anti-PCM1 antibody

Western blot - Anti-PCM1 antibody (AB72443)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (9)

Product promise