AB126142

Anti-PCYT2 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal PCYT2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCYT2 aa 1-300.

대체 명칭 보기

Ethanolamine-phosphate cytidylyltransferase, CTP:phosphoethanolamine cytidylyltransferase, Phosphorylethanolamine transferase, PCYT2

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCYT2 antibody (AB126142)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCYT2 antibody (AB126142)

ab126142 at 1/500 dilution staining PCYT2 in Human Mahlavu (human hepatocellular carcinoma cell line) xenograft tissue by Immunohistochemistry.

Unknown

Western blot - Anti-PCYT2 antibody (AB126142)

10% SDS PAGE

All lanes:

Western blot - Anti-PCYT2 antibody (ab126142) at 1/1000 dilution

All lanes:

HepG2 lysate at 30 µg

Predicted band size: 43 kDa

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CiteAb

Western blot - Anti-PCYT2 antibody (AB126142)

PCYT2 western blot using anti-PCYT2 antibody ab126142. Publication image and figure legend from Chong, U. R., Abdul-Rahman, P. S., et al., 2014, Biomed Res Int, PubMed 24455694.

ab126142 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126142 please see the product overview.

Western blot analyses of NDUFA10, PCYT2, and UQCRC2 of HepG2 cells. (a) Western blot cropped images of NDUFA10, PCYT2, UQCRC2, and beta actin bands detected by antisera against the respective proteins; (b) densitometry analyses of Western blot using ImageJ software. Assay was done in triplicate and data are represented as mean ± standard deviation.

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PCYT2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q99447

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PCYT2 also known as ethanolamine-phosphate cytidylyltransferase is an enzyme that plays a critical role in phospholipid metabolism. It has a molecular mass of approximately 41 kDa. PCYT2 is expressed in various tissues with high levels found in the liver and brain. The enzyme catalyzes the conversion of phosphoethanolamine and CTP to CDP-ethanolamine an important step in the CDP-ethanolamine pathway which synthesizes phosphatidylethanolamine.

Biological function summary

The function of PCYT2 centers around lipid metabolism and membrane biosynthesis. PCYT2 acts as an integral component of the CDP-ethanolamine pathway which is essential for the production of phosphatidylethanolamine a principal phospholipid in cell membranes. It works in conjunction with other enzymes to ensure the proper biosynthesis of phospholipids. Although its precise participation as part of a larger protein complex remains uncertain its enzymatic activity is significant for lipid homeostasis.

Pathways

The activity of PCYT2 mainly fits into the phosphatidylserine decarboxylation and CDP-ethanolamine pathways. The enzyme interacts with CDP-diacylglycerol and phosphatidylcholine playing a significant role in lipid assembly and remodeling of cellular membranes. It shares functional interactions within these pathways with other enzymes like CHPT1 which further converges toward the regulation of phosphatidylcholine and phosphatidylethanolamine synthesis.

Mutations in PCYT2 have been linked to neurodegenerative diseases and certain metabolic disorders. Its dysfunction can contribute to neurological symptoms due to its fundamental role in maintaining normal brain phospholipid levels. The protein's connections to disorders particularly concerning phospholipid imbalance include interactions with related enzymes like EPT1. Such connections underline how PCYT2 disruptions can impact phospholipid biosynthesis and potential disease outcomes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ethanolamine-phosphate cytidylyltransferase that catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PubMed : 31637422, PubMed : 9083101). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PubMed : 9083101).

See full target information PCYT2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 6:300 PubMed36944894

2023

SB2301-mediated perturbation of membrane composition in lipid droplets induces lipophagy and lipid droplets ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjoo Jung,Jongbeom Park,Mingi Kim,Jaeyoung Ha,Hana Cho,Seung Bum Park

Brain : a journal of neurology 142:3382-3397 PubMed31637422

2019

Mutations in PCYT2 disrupt etherlipid biosynthesis and cause a complex hereditary spastic paraplegia.

Applications

Unspecified application

Species

Unspecified reactive species

Frédéric M Vaz,John H McDermott,Mariëlle Alders,Saskia B Wortmann,Stefan Kölker,Mia L Pras-Raves,Martin A T Vervaart,Henk van Lenthe,Angela C M Luyf,Hyung L Elfrink,Kay Metcalfe,Sara Cuvertino,Peter E Clayton,Rebecca Yarwood,Martin P Lowe,Simon Lovell,Richard C Rogers,Antoine H C van Kampen,Jos P N Ruiter,Ronald J A Wanders,Sacha Ferdinandusse,Michel van Weeghel,Marc Engelen,Siddharth Banka

BioMed research international 2013:459017 PubMed24455694

2013

Effects of Tamarindus indica fruit pulp extract on abundance of HepG2 cell lysate proteins and their possible consequential impact on metabolism and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ursula R W Chong,Puteri S Abdul-Rahman,Azlina Abdul-Aziz,Onn H Hashim,Sarni Mat-Junit

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCYT2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCYT2 antibody (AB126142)

Western blot - Anti-PCYT2 antibody (AB126142)

Western blot - Anti-PCYT2 antibody (AB126142)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise