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AB15053

Anti-PCYT2 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal PCYT2 antibody. Suitable for ICC and reacts with Human samples. Cited in 2 publications.
1 이미지
Immunocytochemistry - Anti-PCYT2 antibody (AB15053)
  • ICC

Supplier Data

Immunocytochemistry - Anti-PCYT2 antibody (AB15053)

Immunofluorescence analysis of PCYT2 in Hep G2 cells using ab15053. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab15053 at 5 μg/mL in 0.1% BSA and incubated incubated at 4 degree Celsius overnight followed by a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2000 dilution for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody at 2μg/ml. (Panel d) represents the merged image showing co-localization of PCYT2 with GRP94 in Endoplasmic Reticulum. (Panel e) shows the no primary antibody control. The images were captured at 60X magnification.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC

applications

Immunogen

Synthetic Peptide within Human PCYT2 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q99447

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대체품

Primary Antibodies

AB126142

Anti-PCYT2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCYT2 antibody (AB126142)

  • 0
  • 0

보완 제품

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB123157

Recombinant Human PCYT2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human PCYT2 protein (His tag N-Terminus) (AB123157)

  • 0
  • 0
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
Primary Antibodies

AB134122

Anti-c-Fos antibody [EPR883(2)]

20ul selling size|KO Validated|Recombinant|RabMAb|Lab Essentials

Flow Cytometry (Intracellular) - Anti-c-Fos antibody [EPR883(2)] (AB134122)

  • 1
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "<p></p>" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Primates": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
Preservative: 0.02% Thimerosal (merthiolate) Constituents: 99% Tris glycine, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PCYT2 also known as ethanolamine-phosphate cytidylyltransferase is an enzyme that plays a critical role in phospholipid metabolism. It has a molecular mass of approximately 41 kDa. PCYT2 is expressed in various tissues with high levels found in the liver and brain. The enzyme catalyzes the conversion of phosphoethanolamine and CTP to CDP-ethanolamine an important step in the CDP-ethanolamine pathway which synthesizes phosphatidylethanolamine.
Biological function summary

The function of PCYT2 centers around lipid metabolism and membrane biosynthesis. PCYT2 acts as an integral component of the CDP-ethanolamine pathway which is essential for the production of phosphatidylethanolamine a principal phospholipid in cell membranes. It works in conjunction with other enzymes to ensure the proper biosynthesis of phospholipids. Although its precise participation as part of a larger protein complex remains uncertain its enzymatic activity is significant for lipid homeostasis.

Pathways

The activity of PCYT2 mainly fits into the phosphatidylserine decarboxylation and CDP-ethanolamine pathways. The enzyme interacts with CDP-diacylglycerol and phosphatidylcholine playing a significant role in lipid assembly and remodeling of cellular membranes. It shares functional interactions within these pathways with other enzymes like CHPT1 which further converges toward the regulation of phosphatidylcholine and phosphatidylethanolamine synthesis.

Mutations in PCYT2 have been linked to neurodegenerative diseases and certain metabolic disorders. Its dysfunction can contribute to neurological symptoms due to its fundamental role in maintaining normal brain phospholipid levels. The protein's connections to disorders particularly concerning phospholipid imbalance include interactions with related enzymes like EPT1. Such connections underline how PCYT2 disruptions can impact phospholipid biosynthesis and potential disease outcomes.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Ethanolamine-phosphate cytidylyltransferase that catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PubMed : 31637422, PubMed : 9083101). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PubMed : 9083101).
See full target information PCYT2

대체 명칭 보기

Ethanolamine-phosphate cytidylyltransferase, CTP:phosphoethanolamine cytidylyltransferase, Phosphorylethanolamine transferase, PCYT2
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 3:246 PubMed32427948

2020

Cells grown in three-dimensional spheroids mirror in vivo metabolic response of epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Lagies,Manuel Schlimpert,Simon Neumann,Astrid Wäldin,Bernd Kammerer,Christoph Borner,Lukas Peintner

Cancer research 78:2290-2304 PubMed29358170

2018

2-Hydroxyglutarate-Mediated Autophagy of the Endoplasmic Reticulum Leads to an Unusual Downregulation of Phospholipid Biosynthesis in Mutant IDH1 Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Pavithra Viswanath,Marina Radoul,Jose Luis Izquierdo-Garcia,Wei Qiang Ong,Hema Artee Luchman,J Gregory Cairncross,Bo Huang,Russell O Pieper,Joanna J Phillips,Sabrina M Ronen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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