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AB278010

Anti-PD-L1 antibody [28-8] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal PD-L1 antibody. Carrier free. Suitable for sELISA and reacts with Monkey, Human samples.
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Sandwich ELISA - Anti-PD-L1 antibody [28-8] - BSA and Azide free (Detector) (AB278010)
  • sELISA

Lab

Sandwich ELISA - Anti-PD-L1 antibody [28-8] - BSA and Azide free (Detector) (AB278010)

Sandwich ELISA - Recombinant human PD-L1 protein standard curve.

Background subtracted standard curve using Anti-PD-L1 antibody [EPR23939-25] - BSA and Azide free (Capture), Anti-PD-L1 antibody [28-8] - BSA and Azide free (Detector), and Recombinant human PD-L1 protein (Active) (ab280943) in sandwich ELISA.

관련 conjugated 항체와 다양한 조성의 항체 (13)

  • Unconjugated

    Anti-PD-L1 antibody [28-8]

  • Carrier free

    Anti-PD-L1 antibody [28-8] - BSA and Azide free

  • Carrier free

    Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free

  • 660 APC

    APC Anti-PD-L1 antibody [28-8]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PD-L1 antibody [28-8]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PD-L1 antibody [28-8]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PD-L1 antibody [28-8]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PD-L1 antibody [28-8]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain

  • Biotin

    Biotin Anti-PD-L1 antibody [28-8] (Detector)

  • 519 FITC

    FITC Anti-PD-L1 antibody [28-8]

  • HRP

    HRP Anti-PD-L1 antibody [28-8]

  • 578 PE

    PE Anti-PD-L1 antibody [28-8]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

28-8

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Monkey

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB277712

Human PD-L1 ELISA Kit [28-8]

Recombinant|SimpleStep

ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

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Sample Prep & Detection Kits

AB270705

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (AB270705)

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Sample Prep & Detection Kits

AB270709

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (AB270709)

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Proteins & Peptides

AB280943

Recombinant human PD-L1 protein (Active)

Premium Bioactive

Sandwich ELISA - Recombinant human PD-L1 protein (Active) (AB280943)

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Primary Antibodies

AB201811

Anti-PD1 antibody [NAT105] - BSA and Azide free

BOND RX™ Validated|Recombinant

Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] - BSA and Azide free (AB201811)

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Primary Antibodies

AB281151

Anti-LAG-3 antibody [EPR23884-30] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-LAG-3 antibody [EPR23884-30] - BSA and Azide free (Capture) (AB281151)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Monkey": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab278010 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PD-L1 [28-8]. The recommended pair for sandwich ELISA is:
Capture: ab278009, Human PD-L1 [28-8] Capture Antibody (unconjugated)
Detector: ab278010, Human PD-L1 [28-8] Detector Antibody (unconjugated)
The reference range value is 7.813 - 500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.
Biological function summary

PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.

Pathways

PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.

PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed : 10581077). Can also act as a transcription coactivator : in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed : 32929201).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28813410, PubMed : 28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
See full target information CD274

대체 명칭 보기

CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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