AB324957

Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PD-L2 antibody. Carrier free. Suitable for Flow Cyt (Intra), Flow Cyt and reacts with Transfected cell line - Mouse, Mouse samples.

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Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

This data was developed using ab324944, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T cells transfected with a mouse PDCD1LG2 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling PD-L2 with ab324944 at 1/50000 dilution (0.001ug) / Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control or our antibody. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

This data was developed using ab324944, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse bone marrow derived Dendritic cells induced by 20ng/ml GM-CSF for 13 days and 50ng/ml IL4 for 7 days (Lower left and right) / Mouse bone marrow (Upper left and right) cells labelling PD-L2 with ab324944 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with anti-CD11c conjugated to BV421 and anti-MHCII conjugated to APC/CY7 .
Gated on viable CD11c positive cells.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

This data was developed using ab324944, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell treated with 5ng/ml mouse GM-CSF overnight (Lower left and right) / Untreated control (Upper left and right) cells labelling PD-L2 with ab324944 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were stained with anti-CD3 conjugated to Alexa Fluor®647.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

This data was developed using ab324944, the same antibody clone in a different buffer formulation.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were stained with anti-CD11c conjugated to Brilliant Violet 421.
Gated on viable cells.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30468-530

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt (Intra), Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

ab324957 is the carrier-free version of ab324944

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11224527, PubMed : 11283156, PubMed : 12719480, PubMed : 18641123). Acts as a ligand for the inhibitory receptor PDCD1/PD-1, inhibiting T-cell proliferation by blocking cell cycle progression and cytokine production (PubMed : 11224527, PubMed : 11283156, PubMed : 12719480, PubMed : 18641123).

See full target information Pdcd1lg2

대체 명칭 보기

CD273, B7dc, Btdc, Cd273, Pdl2, Pdcd1lg2, Programmed cell death 1 ligand 2, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

Flow Cytometry - Anti-PD-L2 antibody [EPR30468-530] - BSA and Azide free (AB324957)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise