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AB227829

Anti-PDE10A antibody [EPR22383]

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(5 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal PDE10A antibody. Suitable for IP, WB, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

대체 명칭 보기

PDE10A

8 이미지
Immunoprecipitation - Anti-PDE10A antibody [EPR22383] (AB227829)
  • IP

Unknown

Immunoprecipitation - Anti-PDE10A antibody [EPR22383] (AB227829)

PDE10A was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab227829 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227829 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1 : HeLa whole cell lysate 10 μg (Input).
Lane 2 : ab227829 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab227829 in HeLa whole cell lysate.
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-PDE10A antibody [EPR22383] (ab227829)

Predicted band size: 88 kDa

false

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on corpus striatum in rat brain (PMID : 16864896) is observed. Counterstained with DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on mouse caudate-putamen and globus pallidus in mouse brain (PMID : 16864896; PMID : 28115486) is observed. Counterstained with DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Negative control : No staining on mouse kidney (PMID : 16864896). Counterstained with DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Negative control : No staining on rat kidney (PMID : 16864896). Counterstained with DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PDE10A antibody [EPR22383] (AB227829)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling PDE10A with ab227829 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on corpus striatum in mouse brain (PMID : 16864896) is observed. Counterstained with DAPI (blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Western blot - Anti-PDE10A antibody [EPR22383] (AB227829)
  • WB

Unknown

Western blot - Anti-PDE10A antibody [EPR22383] (AB227829)

Blocking/Dilution buffer : 5% NFDM/TBST.

ab227829 was shown to specifically react with PDE10A in wild-type HAP1 cells as signal was lost in PDE10A knockout cells. Wild-type and PDE10A knockout samples were subjected to SDS-PAGE. ab227829 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

All lanes:

Western blot - Anti-PDE10A antibody [EPR22383] (ab227829) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

PDE10A knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 88 kDa

Observed band size: 88 kDa

false

Exposure time: 3min

Western blot - Anti-PDE10A antibody [EPR22383] (AB227829)
  • WB

Unknown

Western blot - Anti-PDE10A antibody [EPR22383] (AB227829)

Exposure time : Lane 1 : 3 minutes; Lane 2-3 : 3 seconds; Lane 4 : 26 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PDE10A antibody [EPR22383] (ab227829) at 1/1000 dilution

Lane 1:

Human brain lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Mouse testis lysate at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 88 kDa

Observed band size: 88 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (8)

  • Carrier free

    Anti-PDE10A antibody [EPR22383] - BSA and Azide free

  • 660 APC

    APC Anti-PDE10A antibody [EPR22383]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PDE10A antibody [EPR22383]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PDE10A antibody [EPR22383]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PDE10A antibody [EPR22383]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PDE10A antibody [EPR22383]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-PDE10A antibody [EPR22383]

  • 578 PE

    PE Anti-PDE10A antibody [EPR22383]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22383

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-Fr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB151454

Anti-PDE10A antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (AB151454)

  • 4
  • 1

보완 제품

Primary Antibodies

AB154857

Anti-PDE7A/HCP1 antibody [EPR7731]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PDE7A/HCP1 antibody [EPR7731] (AB154857)

  • 0
  • 0
Primary Antibodies

AB171750

Anti-PDE4D antibody [EPR11823-31]

RabMAb|Recombinant

Western blot - Anti-PDE4D antibody [EPR11823-31] (AB171750)

  • 4
  • 1
Primary Antibodies

AB76238

Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (AB76238)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/2000", "IHCFr-species-notes": "<p>Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/2000", "IHCFr-species-notes": "<p>Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 10A (PDE10A) also known as phosphodiesterase 10 is an enzyme that hydrolyzes cyclic nucleotides such as cAMP and cGMP into non-cyclic forms which reduces their signaling effects. PDE10A has a molecular weight of approximately 89 kDa. This enzyme shows higher expression in the brain especially in the striatum a region important for motor control and certain cognitive functions. It occurs in other tissues at lower levels but its role there is less clear.
Biological function summary

PDE10A modulates intracellular signaling pathways critical for neurological processes by controlling levels of cyclic nucleotides. Although PDE10A acts independently it interacts with various signaling molecules affecting neuronal signaling. It exerts significant influence over the dopamine and glutamate neurotransmitter systems important for regulating neuronal excitability and synaptic transmission.

Pathways

Several neurological pathways rely on the function of PDE10A to maintain homeostasis. The enzyme plays a role in the cAMP and cGMP signaling pathways both of which are fundamental in mediating various cellular responses. PDE10A shares functional relationships with other phosphodiesterase family members such as PDE1 and PDE2 which also regulate cyclic nucleotide levels in the central nervous system.

An altered PDE10A function links to several neurological conditions including Huntington's disease and schizophrenia. Dysregulation of dopamine signaling within these disorders implicates PDE10A since it modulates this neurotransmitter pathway. In schizophrenia abnormal PDE10A activity could affect proteins like D2 dopamine receptors contributing to altered signaling in neural circuits associated with this disorder.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides (PubMed : 10373451, PubMed : 10393245, PubMed : 16330539, PubMed : 17389385, PubMed : 27058447). Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate (PubMed : 10373451, PubMed : 10393245, PubMed : 17389385, PubMed : 27058447). May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition (PubMed : 27058447).
See full target information PDE10A
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Medical oncology (Northwood, London, England) 42:456 PubMed40883565

2025

Wedelolactone induces pyroptosis to suppress retinoblastoma by inhibiting the Nrf2/Keap1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Jiang,Hua Jiang,Guitao Wu,Ningdong Pang,Chuanqiang Niu,Zhouping Wang,Haibo Li

Histology and histopathology :18951 PubMed40548384

2025

Loss of PDE10A is associated with metastasis of colon adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sumaita Arowa,Nicholas Nuechterlein,Vinny Ha,Allison Shelbourn,Ashis Chowdhury,Yeshavanth K Banasavadi-Siddegowda,Jerzy Lasota,Markku Miettinen,Patrick J Cimino

Scientific reports 15:18633 PubMed40437028

2025

The relationship between acid-sensing ion channel, ASIC2, and oncogenic β-catenin signaling in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tanvi Joshi,Sagar Chokshi,Annelise Wilhite,Mary Howard Singleton,Elizabeth Catranis,Jennifer Scalici,Kevin J Lee

The international journal of neuropsychopharmacology 27: PubMed39099166

2024

Identification of Phosphodiesterase-7A (PDE7A) as a Novel Target for Reducing Ethanol Consumption in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Wei,Fangjiao Zong,Jiahao Dong,Wei Zhao,Fangfang Zhang,Wei Wang,Shuang Zhao,Ziqi Wang,Fang Zhang,Han-Ting Zhang

Neuropathology and applied neurobiology 47:564-578 PubMed33330988

2021

Effects of mutant huntingtin inactivation on Huntington disease-related behaviours in the BACHD mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Y Cheong,Barbara Baldo,Muhammad U Sajjad,Deniz Kirik,Åsa Petersén
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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