Anti-PDE6 alpha antibody
- BOND RX™ Validated
- 각 태그의 뜻
Be the first to review this product! 리뷰 제출
|
(15 제품이 사용된 논문 )
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 alpha antibody (AB5659)
IHC image of ab5659 staining in human renal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5659, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
- WB
Unknown
Western blot - Anti-PDE6 alpha antibody (AB5659)
Western blot of PDE6 alpha on sheep retinal extracts using ab5659.
All lanes:
Western blot - Anti-PDE6 alpha antibody (ab5659)
Predicted band size: 100 kDa
false
Reactivity 정보
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PDE6 alpha functions within a larger enzyme complex known as the PDE6 holoenzyme. This complex consists of multiple subunits including the PDE6 beta and PDE6 gamma subunits which form the catalytic core. PDE6 alpha contributes to the regulation of cGMP levels which is essential for maintaining the electrical response of photoreceptors to light. The precise action of hydrolyzing cGMP mediates the phototransduction cascade that eventually leads to visual perception.
Pathways
With regard to pathways in cellular function PDE6 alpha is an important player in the phototransduction and visual signal transduction pathways. Within the phototransduction pathway PDE6 alpha interacts closely with other proteins like rhodopsin and transducin. These proteins activate PDE6 alpha upon light exposure initiating the cascade that results in hyperpolarization of the photoreceptor cell membrane. The role of PDE6 alpha in managing cGMP concentration positions it as a major component in these pathways.
제품 프로토콜
- Visit the General protocols
- Visit the Troubleshooting
타겟 정보
대체 명칭 보기
제품이 사용된 논문 (15)
Recent publications for all applications. Explore the 전체 목록 and refine your search
The Journal of biological chemistry 301:108265 PubMed39909376
2025
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 12:20195 PubMed36424523
2022
Applications
Unspecified application
Species
Unspecified reactive species
Cellular and molecular life sciences : CMLS 78:6505-6532 PubMed34420069
2021
Applications
Unspecified application
Species
Unspecified reactive species
The Journal of biological chemistry 291:10501-14 PubMed27008858
2016
Applications
Unspecified application
Species
Unspecified reactive species
Cell communication and signaling : CCS 12:67 PubMed25323447
2014
Applications
WB
Species
Mouse
Nature communications 5:4047 PubMed24915161
2014
Applications
Unspecified application
Species
Unspecified reactive species
Human molecular genetics 23:514-23 PubMed24101599
2013
Applications
WB
Species
Human
Human molecular genetics 22:558-67 PubMed23108158
2012
Applications
WB
Species
Mouse
Proceedings of the National Academy of Sciences of 106:17534-9 PubMed19805139
2009
Applications
IHC-FoFr, WB
Species
Mouse, Mouse
The Journal of neuroscience : the official journal of the Society for Neuroscience 29:9748-60 PubMed19657028
2009
Applications
WB
Species
Mouse
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com