AB5452

Anti-PDGFR alpha (phospho Y742) antibody

(1 리뷰)

(7 제품이 사용된 논문 )

Rabbit Polyclonal PDGFR alpha phospho Y742 antibody. Suitable for WB and reacts with Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human PDGFRA pY742.

1 이미지

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Western blot - Anti-PDGFR alpha (phospho Y742) antibody (AB5452)

Peptide Competition : Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 μg/mL ab5452 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with : no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5452 blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Competition : Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5)

All lanes:

Western blot - Anti-PDGFR alpha (phospho Y742) antibody (ab5452)

Predicted band size: 122 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human PDGFRA pY742. The exact immunogen used to generate this antibody is proprietary information.

P16234

Specificity

PDGFR beta (50% homologous) has not been tested, but is not expected to react.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.35-1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at Tyrosine 742.

보관 버퍼

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR alpha also known as platelet-derived growth factor receptor alpha is a cell surface receptor tyrosine kinase with a mass of approximately 123 kDa. It plays a mechanical role in transmitting signals across the cell membrane initiating cellular responses. PDGFR alpha is widely expressed in mesenchymal cells including fibroblasts and smooth muscle cells. Researchers frequently employ techniques such as PDGFRA IHC and PDGFRA staining to study the localization and expression levels in tissues.

Biological function summary

PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.

Pathways

PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.

PDGFR alpha is implicated in conditions such as gastrointestinal stromal tumors (GISTs) and idiopathic pulmonary fibrosis (IPF). It connects to proteins such as KIT in GISTs where mutations cause aberrant receptor signaling leading to tumorigenesis. Aberrant PDGFR alpha signaling also associates with IPF a disorder characterized by increased fibroblast proliferation and fibrotic tissue formation. These connections make PDGFR alpha a significant target for research and therapeutic intervention.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

See full target information PDGFRA pY742

대체 명칭 보기

CD140a, PDGFR2, RHEPDGFRA, PDGFRA, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 14:4162 PubMed37443109

2023

Inhibition of AXL receptor tyrosine kinase enhances brown adipose tissue functionality in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Vissarion Efthymiou,Lianggong Ding,Miroslav Balaz,Wenfei Sun,Lucia Balazova,Leon G Straub,Hua Dong,Eric Simon,Adhideb Ghosh,Aliki Perdikari,Svenja Keller,Umesh Ghoshdastider,Carla Horvath,Caroline Moser,Bradford Hamilton,Heike Neubauer,Christian Wolfrum

Cell stem cell 28:685-701.e7 PubMed33539723

2021

Pathologic HIF1α signaling drives adipose progenitor dysfunction in obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Mengle Shao,Chelsea Hepler,Qianbin Zhang,Bo Shan,Lavanya Vishvanath,Gervaise H Henry,Shangang Zhao,Yu A An,Yibo Wu,Douglas W Strand,Rana K Gupta

Metabolism: clinical and experimental 103:154014 PubMed31751577

2019

The homeobox factor Irx3 maintains adipogenic identity.

Applications

Unspecified application

Species

Unspecified reactive species

Jan-Inge Bjune,Laurence Dyer,Gro V Røsland,Karl Johan Tronstad,Pål R Njølstad,Jørn V Sagen,Simon N Dankel,Gunnar Mellgren

Cancer & metabolism 7:6 PubMed31164982

2019

Epithelial to mesenchymal transition (EMT) is associated with attenuation of succinate dehydrogenase (SDH) in breast cancer through reduced expression of .

Applications

Unspecified application

Species

Unspecified reactive species

Gro V Røsland,Sissel E Dyrstad,Deusdedit Tusubira,Reham Helwa,Tuan Zea Tan,Maria L Lotsberg,Ina K N Pettersen,Anna Berg,Charlotte Kindt,Fredrik Hoel,Kirstine Jacobsen,Ari J Arason,Agnete S T Engelsen,Henrik J Ditzel,Per E Lønning,Camilla Krakstad,Jean P Thiery,James B Lorens,Stian Knappskog,Karl J Tronstad

Nature communications 9:4583 PubMed30389923

2018

Neomorphic PDGFRA extracellular domain driver mutations are resistant to PDGFRA targeted therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Carman K M Ip,Patrick K S Ng,Kang Jin Jeong,S H Shao,Zhenlin Ju,P G Leonard,Xu Hua,Christopher P Vellano,Richard Woessner,Nidhi Sahni,Kenneth L Scott,Gordon B Mills

Cancer research 73:6219-29 PubMed23970477

2013

Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas.

Applications

Unspecified application

Species

Human

Barbara S Paugh,Xiaoyan Zhu,Chunxu Qu,Raelene Endersby,Alexander K Diaz,Junyuan Zhang,Dorine A Bax,Diana Carvalho,Rui M Reis,Arzu Onar-Thomas,Alberto Broniscer,Cynthia Wetmore,Jinghui Zhang,Chris Jones,David W Ellison,Suzanne J Baker

Stem cells (Dayton, Ohio) 27:116-25 PubMed18403757

2008

Efficient serum-free derivation of oligodendrocyte precursors from neural stem cell-enriched cultures.

Applications

Species

Mouse

Rajesh C Rao,Justin Boyd,Raji Padmanabhan,Josh G Chenoweth,Ronald D McKay

제품이 사용된 논문 모두 보기

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Product promise

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Anti-PDGFR alpha (phospho Y742) antibody

Western blot - Anti-PDGFR alpha (phospho Y742) antibody (AB5452)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (7)

Product promise